This cytochrome was shown to act as the electron donor to a selenatereductase (SeO,2 + 2e 5 SeO32 + H2O). In an in vitro assay (1 ml total volume), 100ul of fully reduced cytochrome was added to 10 ul of purified selenate reductase and880 ul of reaction Buffer. Selenate (SeO42) was added (10 ul) at a final concentrationof 10 µM. Assuming complete oxidation of the cytochrome during selenate reduction,determine the final concentration of selenite (SeO32-) in the reaction chamber?

The objective of this question is to determine the final concentration of selenite in the reaction…

This cytochrome was shown to act as the electron donor to a selenate reductase (Se04²¯ + 2e¯ ⇒ SeO3²- + H₂O). In an in vitro assay (1 ml total volume), 100 ul of fully reduced cytochrome was added to 10 μl of purified selenate reductase and 880 μl of reaction Buffer. Selenate (SeO42-) was added (10 µl) at a final concentration of 10 µM. Assuming complete oxidation of the cytochrome during selenate reduction, determine the final concentration of selenite (SeO3²-) in the reaction chamber?

This cytochrome was shown to act as the electron donor to a selenate reductase (Se04²¯ + 2e¯ ⇒ SeO3²- + H₂O). In an in vitro assay (1 ml total volume), 100 ul of fully reduced cytochrome was added to 10 μl of purified selenate reductase and 880 μl of reaction Buffer. Selenate (SeO42-) was added (10 µl) at a final concentration of 10 µM. Assuming complete oxidation of the cytochrome during selenate reduction, determine the final concentration of selenite (SeO3²-) in the reaction chamber?

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Question

This cytochrome was shown to act as the electron donor to a selenate
reductase (SeO,2 + 2e 5 SeO32 + H2O). In an in vitro assay (1 ml total volume), 100
ul of fully reduced cytochrome was added to 10 ul of purified selenate reductase and
880 ul of reaction Buffer. Selenate (SeO42) was added (10 ul) at a final concentration
of 10 µM. Assuming complete oxidation of the cytochrome during selenate reduction,
determine the final concentration of selenite (SeO32-) in the reaction chamber?

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