7. 116.5 ug of trypsin (MW 23.3 kDa) was mixed in a l ml solution with an excess of a peptide substrate. The reaction is stopped after 10 seconds and the amount of cut peptide is measured to be 3.35 umole. The reaction is repeated with [S] - 0.012 M, and the amount of cut peptide is measured to be 1.175 umole in 10 seconds. a) What is the concentration of enzyme present? b) What is Vm ? c) What is KM ? d) What is kcat ?

7. 116.5 ug of trypsin (MW 23.3 kDa) was mixed in a l ml solution with an excess of a peptide substrate. The reaction is stopped after 10 seconds and the amount of cut peptide is measured to be 3.35 umole. The reaction is repeated with [S] - 0.012 M, and the amount of cut peptide is measured to be 1.175 umole in 10 seconds. a) What is the concentration of enzyme present? b) What is Vm ? c) What is KM ? d) What is kcat ?

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Spectroanalytical Methods

Spectroanalytical methods measure the wavelength and intensity of radiations that are either emitted or absorbed by a molecular or atomic species. These methods are useful for evaluating the electronic or atomic arrangement of the molecules of a given analyte.

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7. 116.5 ug of trypsin (MW 23.3 kDa) was mixed in a 1 ml solution with an excess of a peptide
substrate. The reaction is stopped after 10 seconds and the amount of cut peptide is measured to
be 3.35 umole.
The reaction is repeated with [S] - 0.012 M, and the amount of cut peptide is measured to be
1.175 umole in 10 seconds.
a) What is the concentration of enzyme present?
b) What is Vm ?
c) What is KM ?
d) What is kcat ?

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