You are studying the absorbance properties of the COVID-19 virus. You notice at (4.9500x10^-1) absorbance, the molar absorptivity of the G variant is (4.7887x10^5) M1 cm in hexane at (5.3700x10^2) nm. The concentration was determined to be (3.60x10^-4) M. What is the pathlength of the cuvette in nanometers (nm)? Please do not include units in the answer and present your answer in scientific notation. Note: Your answer is assumed to be reduced to the highest power possible. Your Answer: x10

You are studying the absorbance properties of the COVID-19 virus. You notice at (4.9500x10^-1) absorbance, the molar absorptivity of the G variant is (4.7887x10^5) M1 cm in hexane at (5.3700x10^2) nm. The concentration was determined to be (3.60x10^-4) M. What is the pathlength of the cuvette in nanometers (nm)? Please do not include units in the answer and present your answer in scientific notation. Note: Your answer is assumed to be reduced to the highest power possible. Your Answer: x10

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter32: Radiochemical Methods

Section: Chapter Questions

Problem 32.18QAP: The streptomycin in 500 g of a broth was determined by addition of 1.25 mg of the pure antibiotic...

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The streptomycin in 500 g of a broth was determined by addition of 1.25 mg of the pure antibiotic containing 14C. The specific activity of this preparation was found to be 240 cpm/mg for a 30-min count. From the mixture, 0.112 mg of purified streptomycin was isolated which produced 675 counts in 60.0 min. Calculate the concentration in parts per million streptomycin in the sample.
What are analytes and how can they be used for biochemical recognition?
1) a)What is affinity chromatography and what is it used for? b) A drug called Bamlanivimab is used to treat infections with SARS-CoV-2 (Covid-19).This drug was developed by isolating antibodies from the serum of a patient who recovered from Covid-19.What is possibly on the stationary phase in a column used to isolate antibodies to SARS-CoV-2? How are the antibodies eluted off the column to isolate them for drug development?
You obtained the following raw data when setting up a Biuret standard curve: BSA (mg/ml) Absorbancy 540nm 0 0.158 1 0.210 2 0.260 3 0.305 4 0.360 5 0.410 6 0.455 7 0.510 8 0.530 9 0.550 10 0.554 After blanking against a biuret-dH2O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.251 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).
Calculate the (a) Linearity constant (r), (b) y-intercept, (c) Slope, and (d) Protein content of an unknown sample having an absorbance of 0.325.
50 uL of an aqueous sample of double-stranded DNA is dissolved in 950uL of water. This diluted solution has a maximal absorbance of 0.326 at 260 nm. What isthe concentration of the original (more concentrated) DNA sample, expressed in ug/ul? I would like to know the process of this question. And I was wondering as well if the total diluted volume would be 950ul + 50ul = 1000ul
Most of the ultraviolet absorption of proteins at 280 nm is due to their content of a. Glutamate b. Tryptophan c. Alanine d. Aspartate
A trypsin enzyme assay was performed by mixing 50 µL of trypsin with 950 µL of substrate in a cuvette with a pathlength of 1.00 cm. The absorption coefficient for the product formed by this reaction at 480 nm is 4130 M-'cm'. The rate of change in absorbance at 480 nm was 0.129 abs/min. What is the rate of this reaction in units of µmol/min?
4mL of diluted protein solution was prepared by mixing 200uL of the protein solution with 3.8mL of distilled water. 100uL of this diluted was then mixed with 900uL of Lysozyme solution. Lysozyme activity was measured at 450nm and gave a reading of -0.080 (Abs/min). Calculate the concentration of lysozyme activity in units of abs/min/mL.
In a Bradford assay, 17 pl of a protein isolate sample was diluted by adding 24 pl of water prior to the addition 2.0 mL Bradford reagent, Calculate the protein concentration (in mg/ml) of the original protein isolate sample whose absorbance reading at 595 nm is 0,384 after dilution The protein standard solutions used for the calibration curve of the Bradford assay was constructed using bovine serum albumin (BSA) stock solution whose concentration is 200 ug/mL The concentration and the corresponding absorbance reading at 595 nm of the protein standard solutions are summarized in the table below: Standard soln # 3 6 conc of BSA Kug/mL) 12.00 24.00 36.00 48.00 60.00 Absorbance lat 595 nm 0.000 0.156 0.249 0.328 0.411 0.528 zNote: Final answer format must be x.xox (three decimal places) Round off only in the final answer. Do not round off in the middle of the calculation
You performed a kinetic study of the enzyme lipase and you obtained the following double reciprocal (Lineweaver-Burk) plot: 4 y = 4.3511x + 1.8889 %3D 1 -0.5 -0.3 -0.1 0.1 0.3 0.5 0.7 0.9 1/[S] When you performed the same kinetic study in the presence of an inhibitor, you obtained a new line that had y-intercept 3 and x-intercept -0.3. Based on this information select all correct statements about this inhibitor. This is mixed inhibitor. When this inhibitor is present apparent Km of enzyme decreases. This inhibitor prevents substrate from binding to the enzyme. This is uncompetitive inhibitor. When this inhibitor is present apparent Vmax of enzyme decreases. When this inhibitor is present apparent Km of enzyme increases. This inhibitor can bind to either enzyme or enzyme-substrate complex.
10. Applying the Michaelis-Menten Equation II An enzyme catalyzes the reaction M N. The enzyme is present at a concentration of 1 nM, and the Vmax is 2 µM s. The Km for substrate M is 4 µM. (a) Calculate kcat: (b) What values of Vmax and Km would be observed in the presence of sufficient amounts of an uncompetitive inhibitor to generate an a' of 2.0?