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You are studying the 3-subunit protein shown below, which consists of a 14-kDa subunit bound by a disulfide bond to a 60-kDa subunit that is bound by hydrophobic interactions to a 24-kDa subunit: 14 S-S 60 kDa kDa 24 kDa You perform gel-filtration and SDS-PAGE with and without 2- Mercaptoethanol. Select the most likely outcome of each of the following four experiments: 1. Gel-filtration: [Select ] 2. Gel-filtration with 2-Mercaptoethanol: [Select ] 3. SDS-PAGE: [ Select ] 4. SDS-PAGE with 2-Mercaptoethanol v[ Select ] One band corresponding to a molecular mas Two bands corresponding to 24 kDa and 74 Two bands corresponding to 14 kDa and 84 Three bands corresponding to 14 kDa, 24 kD >

You are studying the 3-subunit protein shown below, which consists of a 14-kDa subunit bound by a disulfide bond to a 60-kDa subunit that is bound by hydrophobic interactions to a 24-kDa subunit: 14 S-S 60 kDa kDa 24 kDa You perform gel-filtration and SDS-PAGE with and without 2- Mercaptoethanol. Select the most likely outcome of each of the following four experiments: 1. Gel-filtration: [Select ] 2. Gel-filtration with 2-Mercaptoethanol: [Select ] 3. SDS-PAGE: [ Select ] 4. SDS-PAGE with 2-Mercaptoethanol v[ Select ] One band corresponding to a molecular mas Two bands corresponding to 24 kDa and 74 Two bands corresponding to 14 kDa and 84 Three bands corresponding to 14 kDa, 24 kD >

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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You are studying the 3-subunit protein shown below, which consists of a 14-kDa subunit bound by a disulfide bond to a 60-kDa subunit that is bound by hydrophobic interactions to a 24-kDa subunit: 14 S-S 60 kDa kDa 24 kDa You perform gel-filtration and SDS-PAGE with and without 2- Mercaptoethanol. Select the most likely outcome of each of the following four experiments: 1. Gel-filtration: [Select ] 2. Gel-filtration with 2-Mercaptoethanol: [ Select ] 3. SDS-PAGE: [ Select ] 4. SDS-PAGE with 2-Mercaptoethanol v [ Select ] One band corresponding to a molecular mass of about 100 kDa Two bands corresponding to 24 kDa and 74 kDa Two bands corresponding to 14 kDa and 84 kDa Three bands corresponding to 14 kDa, 24 kDa, and 60 kDa
Transcribed Image Text:You are studying the 3-subunit protein shown below, which consists of a 14-kDa subunit bound by a disulfide bond to a 60-kDa subunit that is bound by hydrophobic interactions to a 24-kDa subunit: 14 S-S 60 kDa kDa 24 kDa You perform gel-filtration and SDS-PAGE with and without 2- Mercaptoethanol. Select the most likely outcome of each of the following four experiments: 1. Gel-filtration: [Select ] 2. Gel-filtration with 2-Mercaptoethanol: [ Select ] 3. SDS-PAGE: [ Select ] 4. SDS-PAGE with 2-Mercaptoethanol v [ Select ] One band corresponding to a molecular mass of about 100 kDa Two bands corresponding to 24 kDa and 74 kDa Two bands corresponding to 14 kDa and 84 kDa Three bands corresponding to 14 kDa, 24 kDa, and 60 kDa
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