In an experiment of Reverse Phase chromatography using C-18 stationary phase and a mixture of methanol/ethyl acetate/acetic acid, a researcher tried to separate the 3 compounds shown below. Which compound would have the lowest Rf and which the highest Rf in this experiment? Explain your answer and show any relevant structures.

Q: If a single spot divides into 3 unique spots during a paper chromatography experiment,  how many Rf…

A: Here we are required to find number of Rf that can be calculated when a single spot divide into 3…

Q: All three of these are gas chromatography runs from my sample. I'm trying to figure out what it…

A: To find the reason for varied retention times and intensities

Q: Sketch a diagram of the GC data expected from a mixture that is 75% 1-pentanol and 25% 1-propanol.…

A: "GC" typically refers to Gas Chromatography, a widely used analytical technique in chemistry. Gas…

Q: 3. Would you expect an alcohol or an ether to have a higher R¡ value on silica gel thin layer…

A: TLC is an analytical technique which can be used to monitor the organic reaction transformations.…

Q: 4. Chromatograms of compounds A and B were obtained at the same flow rate with two columns of equal…

A: Unadjusted Relative retention time is given as : tr(B)/tr(A)

Q: A particular mixture was seperated into three components usin paper chromatography. the Rf value of…

A: Rf value is the ratio of distance travelled by a given component to the distance travelled by the…

Q: same Rf

A: Paper chromatography is the simplest technique to separate the colored compounds, polar-non polar…

Q: A particular mixture was seperated into three components usin paper chromatography. the Rf value of…

A: In chromatography plate, the mobile phase is the one which carries the component of the mixture with…

Q: In Thin Layer Chromatography，why does the least polar of the analytes （biphenyl）have a higher Rf…

A:

Q: You are attempted to purify a compound via column chromatography. Before starting the process, you…

A:

Q: When using liquid chromatography to separate a mixture containing at least two compounds , A and B.…

A: compound A will elute first, i have discussed the reason for it below

Q: Every spot on a student's TLC plate has an Rf value from 0.1 to 0.2. The student used a 10% ethyl…

A: Rf factor means retardation factor is defined as : Rf = Distance travelled by component/Distance…

Q: A student performed TLC experiment to find out the unknown compound C which was obtained during a…

A: The technique which is commonly used in laboratory for the separation of components of a mixture is…

Q: When naphthalene, butyric acid, and phenyl acetate are separated using dichloromethane as the…

A: The given compounds are naphthalene, butyric acid, and phenyl acetate. The TLC plate separate the…

Q: When separating benzil and benzoin in column chromatography, why do you start with 5% ethyl acetate…

A: The question is based on the concepts of column chromatography. we need to explain for chosen…

Q: In another chromatography experiment, pure chemical “A” leaves a yellow spot with an Rf = 0.49 and…

A:

Q: Q24) Consider the following data obtained from the size exclusion chromatography of polypropylene,…

A: This is question on molecular weight of polymer

Q: During a Thin layer chromatography (TLC) analysis of crystallized  napthalene, What would be the…

A: Interpretation: To explain the effect of addition of acetic acid to crystallized naphthalene during…

Q: While preparing the mobile phase to run a monoamine neurochemical HPLC analysis, you miscalculated…

A: HPLC- High perfomance liquid chromatography is an advanced analytical technique used to identify and…

Q: Which compound elutes first and last in reverse chromatography

A: In reverse phase chromatography, stationary phase is non polar and mobile phase is polar in nature.…

Q: Can the following two molecules be seperated by column chromatography or recrystalization? Explain

A: Recrystallization is used to determine the structure based on the melting point of the crystals.…

Q: Which of the following pairs of compounds would be most easily separated by thin layer…

A: Usually, the polar compounds are easily absorbed on the surface of the adsorbent and thus, lower…

Q: One of the comonly used stationary phases in gas chromatography is (Diphenyl)/(dimethyl)1 - x…

A: We know the methyl /phenyl silicone are an important group of silicone stationary phases. Silicones…

Q: If a 0.0920 g sample of a 1:1 mixture of 4-nitroaniline and 2-nitroaniline was separated by column…

A:

Q: ii) What are the adjusted retention times for components A and B? iv) What is the relative retention…

A: 3 . For component A = 8-1.2=6.8 For component B = 10-1.2 = 8.8 4. Separation factor = 1.29

Q: 14. After a thin layer chromatography experiment, spots of J, Kand Lappeared 10.1, 7.0 and 2.7 cm,…

A:

Q: You are monitoring the reaction A --> B via periodic GC analysis. Initially, you only see one peak…

A: GC analysis is analytical technique by which separation of chemical mixture into its constituents…

Q: a) What would you see if you ran a Thin Layer Chromatography (TLC) on a mixture of the three solids…

A:

Q: What is meant by retention time in gas chromatography.  In what order will they come hexane, ethyl…

A: The process of separation of various constituent of a mixture in zone or in phases is known as…

Q: Suppose your TLC plate looks like the image below (ran 1:1 ratio ethyl acetate/hexane). What is the…

A: Rf = distance travelled by component  divided by distance travelled by solvent .  Rf = 1 cm / 6.8 cm…

Q: A mixture of the follwoing compounds was separated by HPLC with a stationary nonpolar phase and a…

A: The polarity of acid, alkane and ether is in the order: acid > ether > alkane

Q: A single analysis using chromatography produced a chromatogram with three peaks. The peak for…

A: Since you have asked a question with multiple sub-parts, we will solve first three sub-parts for…