A mixture of the follwoing compounds was separated by HPLC with a stationary nonpolar phase and a CH3OH/water mobile phase Which of the follwoing chromatograms would you expevt to botain? Explain your reasoning.

Q: IN GC, What effect does changing from a temperature ramp to an isothermal temperature program have…

A: Gas chromatography is the process of separating compounds in a mixture by injecting a gaseous or…

Q: What factor allows molecules to be separated by chromatography?

A: Chromatography is a method which is used to separate, identify and purify the components of a…

Q: Identify the products on your GC chromatogram using the Rf values of the compounds compared the Rf…

A: Gas chromatography (GC) is a form of analytical chemistry chromatography that is used to separate…

Q: How does the choice of stationary phase in TLC chromatography affect the separation and retention of…

A: Introduction: The selection of the stationary phase in TLC chromatography significantly impacts the…

Q: In kool aid chromatography, why does reducing the dilution of 2-propanol result in its decrease in…

A: In Kool aid chromatography, different colored dyes are separated using a mobile solvent phase and a…

Q: same Rf

A: Paper chromatography is the simplest technique to separate the colored compounds, polar-non polar…

Q: In Thin Layer Chromatography，why does the least polar of the analytes （biphenyl）have a higher Rf…

A:

Q: 1. Why do we use relative and not absolute retention times to characterize separated components?…

A: Retention time refers to the time that is spent by the solute in the stationary phase. The greater…

Q: When using liquid chromatography to separate a mixture containing at least two compounds , A and B.…

A: compound A will elute first, i have discussed the reason for it below

Q: Using reversed phase chromatography in order to separate chloroquine and hydroxychloroquine. Which…

A: Between chloroquine and hydroxy chloroquine, second compound is much more polar than first one. This…

Q: Question 1 Describe in detail the process and mechanism by which thin layer chromatography functions…

A: The question is based on the concept of separation of compounds. thin layer chromatography is a…

Q: Iman has a mixture of two compounds A and B. A is a protein, and B is a lipid. He plans to separate…

A: Apparatus diagram

Q: Q24) Consider the following data obtained from the size exclusion chromatography of polypropylene,…

A: This is question on molecular weight of polymer

Q: Regarding TLC: a) Compounds A and B are analyzed side-by-side by TLC on silica gel. The developed…

A: Thin layer chromatography is a type of adsorption chromatography for the separation of components…

Q: Assume that each compound in the drug mixture would make a spot on the paper the same size as those…

A: The objective of the question is to determine if the resolution of the paper chromatography method…

Q: How does the wavelength in chromatogram affect the signal intensity in an HPLC

A: wavelength in chromatogram affect the signal intensity in an HPLC explanation is given below.

Q: 1. The following are the TLC results from running compounds A and B using column chromatography.…

A: 1. Solution -

Q: You first separated the components of spinach and then identified them by TLC analysis. Was there a…

A: TLC of spinach means separation of chlorophyll pigment.The different form of chlorophyll found in…

Q: During a Thin layer chromatography (TLC) analysis of crystallized  napthalene, What would be the…

A: Interpretation: To explain the effect of addition of acetic acid to crystallized naphthalene during…

Q: Discuss the type of chromatographic system and corresponding reagent

A:

Q: what do the different colors in the result of paper chromatography experiment indicates? 2. Why do…

A: Paper chromatography is a technique in which colour chemicals or substances are separated.

Q: While preparing the mobile phase to run a monoamine neurochemical HPLC analysis, you miscalculated…

A: HPLC- High perfomance liquid chromatography is an advanced analytical technique used to identify and…

Q: Which compound elutes first and last in reverse chromatography

A: In reverse phase chromatography, stationary phase is non polar and mobile phase is polar in nature.…

Q: Doe hydrophobic silica gel act as non-polar stationary phase in TLC making the process as reverse…

A: Reversed  phase chromatography  have  long chain alkyl chains which are non polar in nature .

Q: If a 0.0920 g sample of a 1:1 mixture of 4-nitroaniline and 2-nitroaniline was separated by column…

A:

Q: a) What would you see if you ran a Thin Layer Chromatography (TLC) on a mixture of the three solids…

A:

Q: What is meant by retention time in gas chromatography.  In what order will they come hexane, ethyl…

A: The process of separation of various constituent of a mixture in zone or in phases is known as…

Q: Suppose your TLC plate looks like the image below (ran 1:1 ratio ethyl acetate/hexane). What is the…

A: Rf = distance travelled by component  divided by distance travelled by solvent .  Rf = 1 cm / 6.8 cm…

Q: What is the effect of mobile phase on each (known) sample when doing Gas Chromatography?

A: Gas chromatography (GC) is a special type of chromatographic separation technique that is used to…

Q: Question 6

A: In this titration, hydrochloric acid (HCl) is reacting with sodium hydroxide (NaOH) to form water…

Q: Given that the mobile phase conditions of the experiment use a 70% acetonitrile:30% methanol mixture…

A: Given the mobile phase conditions of the experiment which use a 70% acetonitrile:30% methanol…

Q: Is it easy to difficult to separate p-anisic acid and vanillin by column chromatography? (using…

A: In the given problem we need to discuss whether it is easy to separate p-anisic acid and vanillin by…

Q: 24) The method of internal standard was used to analyze and measure the concentration of chemical X…

A: Volume of unknown X =3.0 mL  Concentration of Y in the mixture = 1.6 x 10-5 M Volume of Y in the…

Q: Given the mass chromatography, identify the peaks of 200, 144, and 115 for 2-Naphthyl-Butylether.

A: Mass spectroscopy is an analytical technique in which the samples are analyzed based on the…

Q: Is it easy to separate benzoic acid and naphthalene by column chromatography? (using ethanol as…

A: Column chromatography is a technique that is used to separate the compounds based on their polarity.…

Q: paper chromatography

A: In this question we have to explain that the arrangement of the above compounds in increasing Rf if…

Q: In an experiment of Reverse Phase chromatography using C-18 stationary phase and a mixture of…

A:

Q: planar chromatography and what type of different planar cgromatographic methods are available

A: The planar chromatography is a technique used to separates mixture of the compounds. The form of…