Ferritin (molecular mass 450 000), transferrin (molecular mass 80 000), and ferric citrate were separated by size exclusion chromatography on Bio-Gel P-300. The column had a length of 37 cm and a 1.5-cm diameter. Eluate fractions of 0.65 ml. were collected. The maximum of each peak came at the following fractions: ferritin, 22; transferrin, 32; and ferric citrate, 84. (That is, the ferritin peak came at an elution volume of 22 x 0.65 mL = 14.3 ml.) Assuming that ferritin is eluted at the interstitial volume and that ferric citrate is eluted at Vy, findK, for transferrin.
Ferritin (molecular mass 450 000), transferrin (molecular mass 80 000), and ferric citrate were separated by size exclusion chromatography on Bio-Gel P-300. The column had a length of 37 cm and a 1.5-cm diameter. Eluate fractions of 0.65 ml. were collected. The maximum of each peak came at the following fractions: ferritin, 22; transferrin, 32; and ferric citrate, 84. (That is, the ferritin peak came at an elution volume of 22 x 0.65 mL = 14.3 ml.) Assuming that ferritin is eluted at the interstitial volume and that ferric citrate is eluted at Vy, findK, for transferrin.
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Transcribed Image Text:Ferritin (molecular mass 450 000), transferrin (molecular mass 80 000), and ferric citrate were separated by size exclusion
chromatography on Bio-Gel P-300. The column had a length of 37 cm and a 1.5-cm diameter. Eluate fractions of 0.65 mL were
collected. The maximum of each peak came at the following fractions: ferritin, 22; transferrin, 32; and ferric citrate, 84. (That is,
the ferritin peak came at an elution volume of 22 x 0.65 mL = 14.3 mL.) Assuming that ferritin is eluted at the interstitial
volume and that ferric citrate is eluted at VM, find Ky for transferrin.
Kay =
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