A single analysis using chromatography produced a chromatogram with three peaks. The peak for compound A has a retention time of 0.44 min, compound B of 5.86 min and compound C of 6.14 min. i) Compound A does not interact at all with the stationary phase. What information can be obtained from the retention time of A? ii) Calculate the retention factors for compounds B and C respectively. iii) Calculate the selectivity factor for compounds B and C.

EBK A SMALL SCALE APPROACH TO ORGANIC L
4th Edition
ISBN:9781305446021
Author:Lampman
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Chapter88: Column Chromatography
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A single analysis using chromatography produced a chromatogram with three
peaks. The peak for compound A has a retention time of 0.44 min, compound
B of 5.86 min and compound C of 6.14 min.
i) Compound A does not interact at all with the stationary phase. What
information can be obtained from the retention time of A?
ii)
Calculate the retention factors for compounds B and C respectively.
iii)
Calculate the selectivity factor for compounds B and C.
iv) The peaks for compounds B and C have respective widths of 0.32 min
and 0.35 min. Calculate the resolution of compounds B and C, and
comment on the significance of the magnitude of your answer.
Transcribed Image Text:A single analysis using chromatography produced a chromatogram with three peaks. The peak for compound A has a retention time of 0.44 min, compound B of 5.86 min and compound C of 6.14 min. i) Compound A does not interact at all with the stationary phase. What information can be obtained from the retention time of A? ii) Calculate the retention factors for compounds B and C respectively. iii) Calculate the selectivity factor for compounds B and C. iv) The peaks for compounds B and C have respective widths of 0.32 min and 0.35 min. Calculate the resolution of compounds B and C, and comment on the significance of the magnitude of your answer.
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