13. Electrospray time-of-flight mass spectrometry was used to analyze the eluate from a high-performance liquid chromatography separation. The mass spectrum of one chromatographic peak, containing a protein of unknown molecular mass, displays MH peaks at m/z= 5 654.208, 5 277.326, 4 947.590, 4 656.613, 4 397.990, and 4 166.576. Find the average molecular mass (M) of the neutral protein and its standard deviation.

13. Electrospray time-of-flight mass spectrometry was used to analyze the eluate from a high-performance liquid chromatography separation. The mass spectrum of one chromatographic peak, containing a protein of unknown molecular mass, displays MH peaks at m/z= 5 654.208, 5 277.326, 4 947.590, 4 656.613, 4 397.990, and 4 166.576. Find the average molecular mass (M) of the neutral protein and its standard deviation.

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

See similar textbooks

Related questions

Q: chromatography on Bio-Gel P-300. The column had a length of 37 cm and a 1.5-cm diameter. Eluate…

A: Molecular mass of transferrin = 80000 Molecular mass of ferritin = 450000 length…

Q: MASS SPECTRUM COMPOUND C Abundance Scan 473 (4.445 min): 311DEHJ.Dldata.ms 2000000 57.1 1900000…

A: Molecular ion peak: Peak of highest mass that corresponds to the MW of molecule Base peak: Peak of…

Q: A peak with a retention time of 407s has a width at half-height of 7.6 seconds. A neighboring peak…

Q: The concentration of Nat in the sea water sample was determined with atomic fluorescence…

A: According to Lamber Beer Law A = €cl € = molal absorptivity coefficient C = concenteration l = path…

Q: MASS SPECTRUM Exepected m/zis 130.231 a. Identify what each fragment listed indiactes. b. What is…

A: The objective of the question is to understand the information provided by the mass spectrum of a…

Q: Cu Absorbance 1 Absorbance 2 Absorbance 3 Mean + SD standard (ppm) 0.0522 0.1013 1 0.0521 0.0520…

A: To determine the concentration of metal in unknown sample, we must first plot the mean absorbance (y…

Q: Loss of which fragment from the parent molecule could account for the peak at 57 in the mass…

A: An analytical technique which is used for the analysis of mass/charge ratios of the fragments…

Q: Mass spectral measurements were made on 10 replicate low concentration Ni-EDTA samples: 178.9,…

A: Given Data:Mass spectral measurements of Ni-EDTA sample are 178.9, 141.2, 158.7, 173.7, 142.7,…

Q: How to interept this data to match the structure? It gives the peak/PPM and having trouble matching…

A: The given NMR data is needed to be interpreted and the each signal obtained is needed to be assigned…

Q: 4. You analyze a mixture of cyclopentane, pyrrolidine, and tetrahydrofuran by gas chromatography and…

A: Gas chromatogram of a mixture of three compounds cyclopentane, pyrrolidine and tetrahydrofuran

Q: Q5. Figure 2 shows the mass spectrum of the same compound from an El source and a Cl source. (a)…

A: a)The best way to figure out the molecular mass of a molecule is to look at its CI spectra. b. The…

Q: A sample of water from the North Sea was analysed by ion-chromatography and the sulfate…

Q: MASS SPECTRUM Exepected m/zis 130.231 a. Identify what each fragment listed indiactes. b. What is…

A: The objective of the question is to understand the information provided by the mass spectrum,…

Q: Please don't use hend raiting and step by step solutions

A: The peak seen at 56, 18 points below the molecular ion peak i at m/z = 74 is enough to identify the…

Q: A spectrophotometer method for the quantitative analysis of Pb2+ in blood uses Cu2+ as an internal…

A: given : SASIS = 0.8320.351= 2.37 CA = concentration of analyte = 1.75 CIS = concentration of…

Q: Mass Spectrum: 100- Relative intensity 80 60 40- 20 10 -----10-7 20 30 40 50 60 70 80 90 100 110 120…

A: Mass spectrometry is one of the best techniques for analyzing the structure of a molecule and…

Q: Find the [FeSCN^2+]eq ε=4.0×10^3 M-1 cm^-1 at 447 nm

A: According to Lambert-Beer Law, “There is a linear relationship between the concentration and the…

Q: Consider the mass spectrum below. 100 80 60 40 20 0.0+ 0.0 40 100 m/z Part 1 Which peak is the…

A: In the mass spectrum, the greatest m/z value is likely to be the molecular ion. some compounds have…

Q: 24) Which one of the given compounds is consistent with the following IR spectrum? MAN

A: Question 24: To identify that the IR spectrum is consistent with which of the given…

Q: How can you distinguish between the given compounds using high-resolution mass spectrometry? 1 O CI…

A: In the given question we have to differentiate between the given compounds using high-resolution…

Q: Below is the mass spectrum and mass data of an unknown compound. 100 70 79 80 98 55 20 66 25 50 75…

A: The determination of the structure of the unknown molecule is accomplished by knowing the molecular…

Q: Mass spectral measurements were made on 10 replicate low concentration Cd-EDTA samples: 181.5,…

Q: Which one of the following techniques cannot be used to measure the amount of Ni in a sample? gas…

A: • Gas chromatogrphy is used to separate different compounds hence it can seprate Ni in a sample. •…

Q: 2. Let's look at the electron-ionization Rel. Intensity 100 80 60 40 20 0.0 10 mass spectra of…

Q: Please help in providing the peaks and what it correlates with of the Carbon NMR spec.

Q: Rel. Intensity 100 80 60 40 20 0.07 0.0 40 51 77 80 m/z 105 Electron impact mass spectrum of ethyl…

A: Given question related to drawing structures of the different fragments of ethyl benzoate in mass…

Q: 10 cm 3 air saturated Fricke dosimetry solution in a 1 cm diameter tube is irradiated for 10 min in…

A: Absorbance is a measure of the quantity of light absorbed by a sample. It can be calculated by using…

Q: The mass spectrum of 2,2-dimethylpropane shows only a very weak molecular ion peak at m/z = 72.…

Q: 18000 10000 14000- 12000 10000 8 8000. 6000- 4000- 2000- 0 0 0.5 Component Acetone 0.82 Isoborneol…

A: Retention time is defined as the time that a solute spends in a column or the time spent in the…

Q: will be the pattern of the molecular ion peak(s) in the mass spectrum of 3-chloro-methyl-benzene…

A: The question is based on the concept of organic chemistry. it involves prediction of peak in mass…

Q: Ferritin (molecular mass 450 000), transferrin (molecular mass 80 000), and ferric citrate were…

A: Fraction of elute = 0.65 mL Elution volume of Ferritin peak = 22 x 0.65 mL = 14.3 mL Length of…

Question

13. Electrospray time-of-flight mass spectrometry was used to analyze the eluate from a high-performance
liquid chromatography separation. The mass spectrum of one chromatographic peak, containing a protein of
unknown molecular mass, displays MH peaks at m/z = 5 654.208, 5 277.326, 4 947.590, 4 656.613, 4
397.990, and 4 166.576. Find the average molecular mass (M) of the neutral protein and its standard deviation.

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

This is a popular solution!

SEE SOLUTION Check out a sample Q&A here

Step 1

Step 2

Step 3

Step 4

Step 5

Step 6

Step 7

Step 8

Trending now

This is a popular solution!

Step by step

Solved in 8 steps with 8 images

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.

Similar questions

1. State the difference between transmittance, absorbance, molar absorptivity, and fluorescence. Which one(s) is proportional to analyte concentration?
A student developed a chromatogram and found that the eluent from travelled 94 mm and the Zn^2+ cation travelled 24 mm. In the development of a chromatogram of a mixture of cations, the eluent front travelled 87 mm. if Zn^2+ is a cation of the unknown mixture, where will its band appear in the chromatogram?
The molecular ion peak, M at m/z 124 and the relative abundance of M, M+1, and M+2 is 100%, 2.21%, and 99.86%. Determine all the possible chemical formulas based on the given information.
The substances in the table below were chromatographed on a gel filtration column. Estimate the molecular weight of the unknown. Compound Blue Dextran 2000 Molecular weight 2 x 106 158 000 V. (mL) 17.7 Aldolase 35.6 Catalase 32.3 210 000 Ferritin 28.6 440 000 Thyroglobulin 25.1 669 000 Unknown 30.3
Polarities of analyte functional group increase in the order of hydrocarbon < ethers < esters < ketones < aldehydes < amides < amines < alcohols Arrange the order of elution [from shortest to longest retention time] for the following six compounds from an HPLC C18 column. C2H5OH CH3COCH3 CH3COOH CH3COOC2H5 C2H50C2H5 (СН3)2NH O A. C2H5OH < CH3COOH < (CH3)2NH < CH3COCH3 < CH3COOC2H5
A
Provide a structure of the fragment in the mass spec producing the peak at 139 m/z and a structure for the fragment that was lost.
Identify the following in the given IR Mass spectrum. Compound 1 Mass Spectroscopy: Interpretation of peaks: Compound 1 Mass Spectroscopy: MASS SPECTRUM 100 80 60 40 20 0.0- 10 20 30 40 50 60 70 m/z Interpretation of peaks: Rel. Intensity
MASS SPECTRUM Exepected m/zis 130.231 a. Identify what each fragment listed indiactes. b. What is the expected relative height of (M+ 1) +