chromatography on Bio-Gel P-300. The column had a length of 37 cm and a 1.5-cm diameter. Eluate fractions of 0.65 mL were collected. The maximum of each peak came at the following fractions: ferritin, 22; transferrin, 32; and ferric citrate, Ferritin (molecular mass 450 000), transferrin (molecular mass 80 000), and ferric citrate were separated by size exclusion 84. (That is, the ferritin peak came at an elution volume of 22 × 0.65 mL = 14.3 mL.) Assuming that ferritin is eluted at the interstitial volume and that ferric citrate is eluted at Vm, find Kay for transferrin. Kav %3D

chromatography on Bio-Gel P-300. The column had a length of 37 cm and a 1.5-cm diameter. Eluate fractions of 0.65 mL were collected. The maximum of each peak came at the following fractions: ferritin, 22; transferrin, 32; and ferric citrate, Ferritin (molecular mass 450 000), transferrin (molecular mass 80 000), and ferric citrate were separated by size exclusion 84. (That is, the ferritin peak came at an elution volume of 22 × 0.65 mL = 14.3 mL.) Assuming that ferritin is eluted at the interstitial volume and that ferric citrate is eluted at Vm, find Kay for transferrin. Kav %3D

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Question

Ferritin (molecular mass 450 000), transferrin (molecular mass 80 000), and ferric citrate were separated by size exclusion
chromatography on Bio-Gel P-300. The column had a length of 37 cm and a 1.5-cm diameter. Eluate fractions of 0.65 mL
were collected. The maximum of each peak came at the following fractions: ferritin, 22; transferrin, 32; and ferric citrate,
84. (That is, the ferritin peak came at an elution volume of 22 x 0.65 mL = 14.3 mL.) Assuming that ferritin is eluted at
the interstitial volume and that ferric citrate is eluted at VM, find Kay for transferrin.
Kay =

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