In the Thermo Fisher application note about wine analysis (Lesson 3), the following chromatogram was collected of nine components of wine. If peak 3 has a retention time of 3.15 minutes and a peak width of 0.070 minutes, and peak 4 has a retention time of 3.24 minutes and a peak width of 0.075 minutes, what is the resolution factor between the two peaks? [Hint: it will help to review Lesson 2 for this question.] mAU 300 200 100 0 3.22 0.62 1.04 1.24 2.4 2.6 2.8 LO 34 12 ww 3.0 5 3.2 3.4 3.6 3.8 4.0 Minutes 7 8 9 4.2 4.4 4.6 4.8 5.0 5.2

In the Thermo Fisher application note about wine analysis (Lesson 3), the following chromatogram was collected of nine components of wine. If peak 3 has a retention time of 3.15 minutes and a peak width of 0.070 minutes, and peak 4 has a retention time of 3.24 minutes and a peak width of 0.075 minutes, what is the resolution factor between the two peaks? [Hint: it will help to review Lesson 2 for this question.] mAU 300 200 100 0 3.22 0.62 1.04 1.24 2.4 2.6 2.8 LO 34 12 ww 3.0 5 3.2 3.4 3.6 3.8 4.0 Minutes 7 8 9 4.2 4.4 4.6 4.8 5.0 5.2

Chemical Principles in the Laboratory

11th Edition

ISBN:9781305264434

Author:Emil Slowinski, Wayne C. Wolsey, Robert Rossi

Publisher:Emil Slowinski, Wayne C. Wolsey, Robert Rossi

Chapter2: Resolution Of Matter Into Pure Substances, I.paper Chromatography

Section: Chapter Questions

Problem 1ASA

See similar textbooks

Similar questions

Mass spectrometry is an extremely versatile detection system for GC. However, interfacing an HPLC system to a mass spectrometer is a much more difficult task. Describe the major reasons why it is more difficult to combine HPLC with mass spectrometry than it is to combine GC with mass spectrometry.
QUESTION 5 In a chromatographic separation using a polar stationary phase and a mobile phase composed of 60% hexane and 40% ethyl acetate, two compounds, benzene (peak A) and toluene (peak B), are eluted. The width of peak A at its base is 1.2 min, the width of peak B at its base is 1.3 min, and the retention time of peak A is 5.2 min and peak B is 3.5 min. What is the resolution (Rs) between these two peaks and is it acceptable? R = 1.36. It is not an acceptable resolution. R = 0.74. It is an acceptable resolution. R = 1.36. It is an acceptable resolution. R = 0.74. It is not an acceptable resolution.
1) Study the chromatograph (below) of a mixture of Compounds A and B, run on the GCs in the teaching labs at CU Boulder. Compound A has the shorter retention time. STAAT 61 1.11 227 RT TYPE AREA XXXX XXXX XXXX AREAS 0.009 55874 44.117 ARIHT 0.61 XX XX XX 1.11 2.27 XX XX XX TOTAL AREA=XX MUL FACTOR=XX 1. What is the retention time of compound A? Compound B? 2. Which compound is present in a larger amount? 3. Which compound has the lower boiling point? 4. What would happen to the retention times of compounds A and B if the column temperature were raised? 5. You suspect that compound B is octane. What can you do to provide supporting evidence for this hypothesis?
A young researcher set out to develop a method for quantifying the polycyclic aromatic hydrocarbon (PAH) content in a contaminated soil sample using open tubular gas chromatography. During the initial stages of method development, the researcher was unable to separate the early eluting compounds, anthracene and naphthalene, with baseline resolution. Select the possible modifications the researcher could make to improve the resolution. use a column with a smaller inner diameter decrease the column length increase the stationary phase thickness increase the temperature choose a different stationary phase
Consider the peaks for heptane (14.56 min) and p-difluorobenzene (14.77 min) in the chromatogram. The column is 30.0 m long. (a) Measure w1/2 for each peak and calculate the number of plates and the plate height for each. (b) From w1/2, compute w for each peak and find the resolution between the two peaks.
Study the chromatograph (below) of a mixture of Compounds A and B, run on the GCs in the teaching labs at CU Boulder. Compound A has the shorter retention time. You suspect that compound B is octane. What can you do to provide supporting evidence for this hypothesis?
In lon Chromatography retention times and the resolution between adjacent peaks can be affected by changing the concentration of the mobile phase. In the ICS2000 instrument used here, the mobile phase is potassium hydroxide. Which ONE of the following responses best explains the effect of increasing the mobile phase concentration on the peak retention times and the resolution between adjacent peaks? As the mobile phase hydroxide concentration is increased the peak retention times decrease and the resolution between adjacent peaks decreases As the mobile phase hydroxide concentration is increased the peak retention times increase and the resolution between adjacent peaks decreases As the mobile phase hydroxide concentration is increased the peak retention times increase and the resolution between adjacent peaks decreases As the mobile phase hydroxide concentration is increased the peak retention times increase and the resolution between adjacent peaks increases
A3
Internal standard. A solution containing 3.47 mM X (analyte) and 1.72 mM S (standard) gave peak areas of 3 473 and 10 222, respectively, in a chromatographic analysis. Then 1.00 mL of 8.47 mM S was added to 5.00 mL of unknown X, and the mixed solution was diluted to 10.0 mL. This solution gave peak areas of 5 428 and 4 431 for X and S, respectively. (a) Find the response factor for X relative to S in Equation 5-9. (b) Find [S] (mM) in the 10.0 mL of mixed solution. (c) Find [X] (mM) in the 10.0 mL of mixed solution.(d) Find [X] in the original unknown.
In molecular exclusion (size exclusion) chromatography, the molecules have the longest retention times. a) the larger molecules b) the smaller molecules
A GC-MS was used to analyze an unknown sample. Based off the chromatogram, at least how many different compounds were in this sample? Detector Signal u M Retention Time
A solution was prepared by mixing 12.49 mg of B plus 10.00 mL of unknown containing A and just diluting to 25.00 mL. Peak areas of 5.97 and 6.38 were observed for A and B, respectively. Find the concentration of A (mg/L) in the unknown.