In the Thermo Fisher application note about wine analysis (Lesson 3), the followingchromatogram was collected of nine components of wine. If peak 3 has a retention time of3.15 minutes and a peak width of 0.070 minutes, and peak 4 has a retention time of 3.24minutes and a peak width of 0.075 minutes, what is the resolution factor between the twopeaks? [Hint: it will help to review Lesson 2 for this question.]MAU300200T345100-1 2CO678902.42.62.83.0 3.2 3.43.63.8 4.0 4.24.44.64.85.05.2Minutes3.220.621.04O 1.24

Answered: In the Thermo Fisher application note…

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter6: Random Errors In Chemical Analysis

Section: Chapter Questions

Problem 6.15QAP

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In the Thermo Fisher application note about wine analysis (Lesson 3), the following chromatogram was collected of nine components of wine. If peak 3 has a retention time of 3.15 minutes and a peak width of 0.070 minutes, and peak 4 has a retention time of 3.24 minutes and a peak width of 0.075 minutes, what is the resolution factor between the two peaks? [Hint: it will help to review Lesson 2 for this question.] mAU 300 200 100 0 3.22 0.62 1.04 1.24 2.4 2.6 2.8 LO 34 12 ww 3.0 5 3.2 3.4 3.6 3.8 4.0 Minutes 7 8 9 4.2 4.4 4.6 4.8 5.0 5.2
QUESTION 5 In a chromatographic separation using a polar stationary phase and a mobile phase composed of 60% hexane and 40% ethyl acetate, two compounds, benzene (peak A) and toluene (peak B), are eluted. The width of peak A at its base is 1.2 min, the width of peak B at its base is 1.3 min, and the retention time of peak A is 5.2 min and peak B is 3.5 min. What is the resolution (Rs) between these two peaks and is it acceptable? R = 1.36. It is not an acceptable resolution. R = 0.74. It is an acceptable resolution. R = 1.36. It is an acceptable resolution. R = 0.74. It is not an acceptable resolution.
A young researcher set out to develop a method for quantifying the polycyclic aromatic hydrocarbon (PAH) content in a contaminated soil sample using open tubular gas chromatography. During the initial stages of method development, the researcher was unable to separate the early eluting compounds, anthracene and naphthalene, with baseline resolution. Select the possible modifications the researcher could make to improve the resolution. use a column with a smaller inner diameter decrease the column length increase the stationary phase thickness increase the temperature choose a different stationary phase
Question: Can high-performance liquid chromatography (HPLC) effectively separate and quantify trace levels of multiple pesticide residues in complex food matrices?
1) Study the chromatograph (below) of a mixture of Compounds A and B, run on the GCs in the teaching labs at CU Boulder. Compound A has the shorter retention time. STAAT 61 1.11 227 RT TYPE AREA XXXX XXXX XXXX AREAS 0.009 55874 44.117 ARIHT 0.61 XX XX XX 1.11 2.27 XX XX XX TOTAL AREA=XX MUL FACTOR=XX 1. What is the retention time of compound A? Compound B? 2. Which compound is present in a larger amount? 3. Which compound has the lower boiling point? 4. What would happen to the retention times of compounds A and B if the column temperature were raised? 5. You suspect that compound B is octane. What can you do to provide supporting evidence for this hypothesis?
In lon Chromatography retention times and the resolution between adjacent peaks can be affected by changing the concentration of the mobile phase. In the ICS2000 instrument used here, the mobile phase is potassium hydroxide. Which ONE of the following responses best explains the effect of increasing the mobile phase concentration on the peak retention times and the resolution between adjacent peaks? As the mobile phase hydroxide concentration is increased the peak retention times decrease and the resolution between adjacent peaks decreases As the mobile phase hydroxide concentration is increased the peak retention times increase and the resolution between adjacent peaks decreases As the mobile phase hydroxide concentration is increased the peak retention times increase and the resolution between adjacent peaks decreases As the mobile phase hydroxide concentration is increased the peak retention times increase and the resolution between adjacent peaks increases
Find the initial concentration of the unknown. Chromotography graph: What is the value of the response factor, F? What is the concentration of the analyte in the unknown?
1,3,5 what the correct answer?
Study the chromatograph (below) of a mixture of Compounds A and B, run on the GCs in the teaching labs at CU Boulder. Compound A has the shorter retention time. You suspect that compound B is octane. What can you do to provide supporting evidence for this hypothesis?
The options are larger or smaller.
A GC-MS was used to analyze an unknown sample. Based off the chromatogram, at least how many different compounds were in this sample? Detector Signal u M Retention Time
The figure below shows a portion of a GC chromatogram for a mixture of two aromatic compounds labeled A and B. The separation employed a 2.50 meter packed column under isothermal conditions (90 °C) and a flow rate of 10 mL/min. Detector Signal 300.0 250.0 200.0 150.0 100.0 50.0 0.0 1.0 1.1 Unretained 1.5 time (min) Find the times that components A and B spend in the stationary phase. 2.0

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