Consider the following setup of a capillary electrophoresis (CE) system: What are the two major factors contributing to the moving speed of molecules in a CE separation process? Does the analyte need to be charged for CE separation? Which type of analytes will reach the detector first, positively-charged or negatively-charged?

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  1. Consider the following setup of a capillary electrophoresis (CE) system:

What are the two major factors contributing to the moving speed of molecules in a CE separation process? Does the analyte need to be charged for CE separation?

Which type of analytes will reach the detector first, positively-charged or negatively-charged?        

The internal surface of the capillary is negatively charged
Anode
Cathode
↑
↑
Injector
Detector
Transcribed Image Text:The internal surface of the capillary is negatively charged Anode Cathode ↑ ↑ Injector Detector
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