Match the following types of column chromatography with the underlying principle. Affinity chromatography HPLC lon-exchange chromatography (EX) Size-exclusion chromatography ✓[Choose] exploits differences in molecular size of individual proteins exploits differences in the size and magnitude of the net charge of individual proteins at specific pis requires a ligand (specific binding partner) to attract and retain a target protein relies on high pressure pumps that force proteins through a column [Choose] [Choose]

Match the following types of column chromatography with the underlying principle. Affinity chromatography HPLC lon-exchange chromatography (EX) Size-exclusion chromatography ✓[Choose] exploits differences in molecular size of individual proteins exploits differences in the size and magnitude of the net charge of individual proteins at specific pis requires a ligand (specific binding partner) to attract and retain a target protein relies on high pressure pumps that force proteins through a column [Choose] [Choose]

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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K 32 Uses a lamp and a flame for determining the concentrations of metal ions in solutions. a. High performance liquid chromatography b. gas chromatography c. mass spectrometry d. infrared spectroscopy e. UV/VIS spectroscopy f. atomic absorption spectroscopy g. thin layer chromatography
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please solve question 10
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Classify the descriptions based on whether they apply to ion‑exchange, size‑exclusion, or both types of chromatography columns. - Proteins move through the column at rates determined by their net charge. - The gel bed will contain polymer beads with charged functional groups. - Proteins move through the column at rates determined by their size. - The gel bed will contain porous polymer beads. - The properties of the proteins affect the rate at which they migrate. - Proteins are separated based on differences in their amino acid sequences.
The figure below shows a typical separation of several fat-soluble vitamins by HPLC. The x-axis is retention time in minutes. From the graph try to estimate retention times for peaks 2 and 3. Retention time peak 2 = Retention time peak 3 = Assume that w1/2 is 0.15 minutes for peak 2 and 0.16 minutes for peak 3. Assume that tm is 2.00 minutes. How much time does gamma-tocopherol spend in the stationary phase? From tr and w1/2 find N for the peak that elutes first. From tr and w1/2 find the resolution of peaks 2 and 3 Calculate α for these peaks. If the flow rate for the column is 1.2 mL/minute what is the retention volume for peak 4?
4
You run the chromatography system with paper as the stationary phase and 70% isopropanol as the mobile phase. You determine the Rf of a polar analyte to be 0.40. If you use 100% isopropanol (a less polar solvent) instead of isopropanol as the mobile phase what would you expect to happen to the Rf value? No value effect on Rf higher lower could be higher or lower
2
What kind of chromatography would you for purification of full length antibody ?
What is the best Chromatography instrument for analysis of Soluble Protein molecules CG-FID GC-MS LC-MS IC-MS O IC-EC
Please explain both answer Correctly step by step