The principle of affinity chromatography is based on: a) Charge interactions b) Size exclusion c) Hydrophobic interactions d) Specific binding interactions between molecules

Match the following types of column chromatography with the underlying principle. Affinity chromatography HPLC lon-exchange chromatography (IEX) Size-exclusion chromatography ✓[Choose ] exploits differences in molecular size of individual proteins exploits differences in the size and magnitude of the net charge of individual proteins at specific pris requires a ligand (specific binding partner) to attract and retain a target protein relies on high pressure pumps that force proteins through a column [Choose] [Choose ]

When would you use flame ionisation detectors in chromatographic analysis?  (a) analysis of hydrocarbons at trace levels (b) Analysis of organic and inorganic compounds at normal concentration levels (c) Analysis of samples that you want to preserve (d) Analysis of hydrocarbons at trace levels and analysis of samples that do not need to be preserved (e) None of the other answers   Pick one and why...

In which mode of chromatography do solute and solvent molecules migrate in and out the stationary phase based on the solute’s equilibrium between the stationary and mobile phases. a) adsorption b) partition c) extraction d) absorbance

Classify the descriptions based on whether they apply to ion‑exchange, size‑exclusion, or both types of chromatography columns. - Proteins move through the column at rates determined by their net charge. - The gel bed will contain polymer beads with charged functional groups. - Proteins move through the column at rates determined by their size. - The gel bed will contain porous polymer beads. - The properties of the proteins affect the rate at which they migrate. - Proteins are separated based on differences in their amino acid sequences.

1) Study the chromatograph (below) of a mixture of Compounds A and B, run on the GCs in the teaching labs at CU Boulder. Compound A has the shorter retention time. STAAT 61 1.11 227 RT TYPE AREA XXXX XXXX XXXX AREAS 0.009 55874 44.117 ARIHT 0.61 XX XX XX 1.11 2.27 XX XX XX TOTAL AREA=XX MUL FACTOR=XX 1. What is the retention time of compound A? Compound B? 2. Which compound is present in a larger amount? 3. Which compound has the lower boiling point? 4. What would happen to the retention times of compounds A and B if the column temperature were raised? 5. You suspect that compound B is octane. What can you do to provide supporting evidence for this hypothesis?

Which of the following are characteristics of normal-phase chromatography?  I - The stationary phase is polar.  II - The mobile phase is more polar than the stationary phase.  III - The eluent strength increases as the mobile phase becomes less polar.  IV - The eluent strength increases as the mobile phase becomes more polar.  V - the stationary phase is nonpolar.    Answers to choose from:  I and IV I, IV and V I and III II and IV II and V ...and why?

A young researcher set out to develop a method for quantifying the polycyclic aromatic hydrocarbon (PAH) content in a contaminated soil sample using open tubular gas chromatography. During the initial stages of method development, the researcher was unable to separate the early eluting compounds, anthracene and naphthalene, with baseline resolution. Select the possible modifications the researcher could make to improve the resolution. use a column with a smaller inner diameter decrease the column length increase the stationary phase thickness increase the temperature choose a different stationary phase

Which of the following types of liquid chromatography uses immobilized biochemical as a stationary phase? affinity chromatography ion exchange chromatography gel permeation chromatography exclusion chromatography

1. From spinach, the chromatographic analysis will yield two major substances: (a) B-carotene (C40H56), which will separate from the spinach mixture to form a yellow band, and (b) chlorophyll A (CSSH72MGN4OS), which will separate from the mixture to form a green band. i. Describe the characteristics and draw structures of these molecules. ii. From the spectrophotometric analysis, what wavelength (Amax) would you expect ß-carotene (C40H56) to absorb? What wavelength would chlorophyll A (CssH72MgN4O5) absorb?