The isoelectric point (pl) of a peptide is the pH at which the peptide ceases to migrate in an electric field. At this pH, a peptide is zwitterionic, meaning it has the same number of positive and negative charges, making it electrically neutral. The pl can be estimated within 0.1 or 0.2 pH units from the pK₁ values of all the proton dissociable groups in the peptide. The table shows the pK, values for side-chain ionizable groups, as well as the peptide terminal amino and carboxyl groups. charge: I 3.4 12.5 8.0 8.2 Determine the total positive charge on the peptide GARAIA when all acidic and basic groups are fully protonated. Enter your answer without the sign. Note that the letters in the peptide name represent one-letter amino acid abbreviations. Enter numeric value Select the pKa values used to estimate the pl. Group aspartate glutamate histidine cysteine tyrosine lysine arginine peptide amino group peptide carboxyl group pK₂ 3.7 4.3 6.0 8.2 10.1 10.5 12.5 8.0 3.4
Neutral Amino Acids
Amino acids which do not have any charge on them are neutral amino acids.
Globular Protein
The globular proteins refer to the shape of protein specifically spherical in nature apart from spherical form fibrous, disordered and membrane-bound proteins exist. These globular proteins are miscible in water and form a colloidal solution rather than other types which might not exhibit solubility. Many classes of the fold are found in globular proteins, which render them a sphere shape. Globular fold containing proteins usually are referred to by the term globin.
Dimer
Dimers are basic organic compounds, which are derivates of oligomers. It is formed by the combination of two monomers which could potentially be strong or weak and in most cases covalent or intermolecular in nature. Identical monomers are called homodimer, the non-identical dimers are called heterodimer. The method by which dimers are formed is known as “dimerization”.
Dipeptide
A dipeptide is considered a mixture of two distinct amino acids. Since the amino acids are distinct, based on their composition, two dipeptide's isomers can be produced. Various dipeptides are biologically essential and are therefore crucial to industry.
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