The isoelectric point, pl, of the protein beta-glucuronidase is 5.1, while that of ribonuclease T1 is 2.9. What is the net charge of beta-glucuronidase at pH 5.7 ?[ What is the net charge of ribonuclease T1 at pH 1.5 ?[ positive negative
Neutral Amino Acids
Amino acids which do not have any charge on them are neutral amino acids.
Globular Protein
The globular proteins refer to the shape of protein specifically spherical in nature apart from spherical form fibrous, disordered and membrane-bound proteins exist. These globular proteins are miscible in water and form a colloidal solution rather than other types which might not exhibit solubility. Many classes of the fold are found in globular proteins, which render them a sphere shape. Globular fold containing proteins usually are referred to by the term globin.
Dimer
Dimers are basic organic compounds, which are derivates of oligomers. It is formed by the combination of two monomers which could potentially be strong or weak and in most cases covalent or intermolecular in nature. Identical monomers are called homodimer, the non-identical dimers are called heterodimer. The method by which dimers are formed is known as “dimerization”.
Dipeptide
A dipeptide is considered a mixture of two distinct amino acids. Since the amino acids are distinct, based on their composition, two dipeptide's isomers can be produced. Various dipeptides are biologically essential and are therefore crucial to industry.
![**Electrophoresis and Isoelectric Points Exercise**
The isoelectric point (pI) of the protein **neuraminidase** is 5.1, while that of **alkaline phosphatase** is 5.7.
1. **Question 1**: What is the net charge of neuraminidase at pH 3.5?
- Options: Positive, Negative
2. **Question 2**: What is the net charge of alkaline phosphatase at pH 7.5?
- Options: Positive, Negative
The isoelectric point of **tyrosine** is 5.66, and for **glutamic acid**, it is 3.22.
3. **Question 3**: During paper electrophoresis at pH 4.5, toward which electrode does tyrosine migrate?
- Options: [Dropdown not visible]
4. **Question 4**: During paper electrophoresis at pH 6, toward which electrode does glutamic acid migrate?
- Options: [Dropdown not visible]
**Buttons:**
- **Submit Answer**
- **Retry Entire Group**
- Note: 2 more group attempts remaining.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F33cbf6b6-7e3c-402e-b2a0-94a8d5e85103%2Fd5121788-f040-4fbf-a814-8395aa24e0c5%2F5rzpnpl_processed.png&w=3840&q=75)
Trending now
This is a popular solution!
Step by step
Solved in 3 steps
