The isoelectric point of a polypeptide or protein is equal to the pH at which the overall charge is equal to zero (0). 1 i Find the pH range to within 1 pH unit over which the polypeptide below will have an overall charge of 0. (In class on Friday, I worked out the changes in charge that the N-terminal -NH3+ and the C-terminal -COOH groups undergo over a limited pH range. Follow through to apply the same approach to all of the ionizing or dissociating groups, including the N-terminal and C-terminal groups, to estimate their separate, individual contributions to the overall charge on the polypeptide as a function of pH. 6, identify the two residues that control the transition for the polypeptide to acquire an overall charge of 0.) Alanyl-cysteinyl-lysyl-glutamyl-asparaginyl-histidinyl-tyrosine Ala - Cys A C *H3N. CH3 H SH - Lys - Glu - Asn His - Tyr K E N H Y HO. H H ■NH3* | NH₂ HNT NH H Гон JOH Ka values 7.0 8.4 10.5 4.1 6.0 4.0 10.5 ΣZavg

The isoelectric point of a polypeptide or protein is equal to the pH at which the overall charge is equal to zero (0). 1 i Find the pH range to within 1 pH unit over which the polypeptide below will have an overall charge of 0. (In class on Friday, I worked out the changes in charge that the N-terminal -NH3+ and the C-terminal -COOH groups undergo over a limited pH range. Follow through to apply the same approach to all of the ionizing or dissociating groups, including the N-terminal and C-terminal groups, to estimate their separate, individual contributions to the overall charge on the polypeptide as a function of pH. 6, identify the two residues that control the transition for the polypeptide to acquire an overall charge of 0.) Alanyl-cysteinyl-lysyl-glutamyl-asparaginyl-histidinyl-tyrosine Ala - Cys A C H3N. CH3 H SH - Lys - Glu - Asn His - Tyr K E N H Y HO. H H ■NH3 | NH₂ HNT NH H Гон JOH Ka values 7.0 8.4 10.5 4.1 6.0 4.0 10.5 ΣZavg

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Spectroanalytical Methods

Spectroanalytical methods measure the wavelength and intensity of radiations that are either emitted or absorbed by a molecular or atomic species. These methods are useful for evaluating the electronic or atomic arrangement of the molecules of a given analyte.

Question

The isoelectric point of a polypeptide or protein is equal to the pH at which the overall charge
is equal to zero (0).
1
i
Find the pH range to within 1 pH unit over which the polypeptide below will have an overall
charge of 0.
(In class on Friday, I worked out the changes in charge that the N-terminal -NH3+ and the C-terminal
-COOH groups undergo over a limited pH range. Follow through to apply the same approach to all of
the ionizing or dissociating groups, including the N-terminal and C-terminal groups, to estimate their
separate, individual contributions to the overall charge on the polypeptide as a function of pH.
6, identify the two residues that control the transition for the polypeptide to
acquire an overall charge of 0.)
Alanyl-cysteinyl-lysyl-glutamyl-asparaginyl-histidinyl-tyrosine
Cys
-
Lys
-
Glu
Ala
-
A C
*H3N
CH3
.SH
H
K
H
HO.
E
-
Asn
His
-
Tyr
N
H
Y
O=0
■NH3*
NH₂
ΤΗΝ
NH
H
Тон
JOH
oka values
7.0
8.4
10.5
4.1
6.0
4.0 10.5
ΣZavg

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