The isoelectric point, pI, of the protein trypsin is 10.5, while that of glyceraldehyde-3-phosphate dehydrogenase is 6.55.- What is the net charge of trypsin at pH 5.1? [Dropdown for selection]- What is the net charge of glyceraldehyde-3-phosphate dehydrogenase at pH 6.1? [Dropdown for selection]The isoelectric point of glutamic acid is 3.22; asparagine is 5.41.- During paper electrophoresis at pH 6, toward which electrode does glutamic acid migrate? [Dropdown for selection]- During paper electrophoresis at pH 6.5, toward which electrode does asparagine migrate? [Dropdown for selection]The text requires an understanding of the relationship between pH and isoelectric point to determine the net charge and migration direction during electrophoresis.

Answered: The isoelectric point, pl, of the…

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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The isoelectric point, pI, of the protein trypsin is 10.5, while that of glyceraldehyde-3-phosphate dehydrogenase is 6.55.

- What is the net charge of trypsin at pH 5.1? [Dropdown for selection]
- What is the net charge of glyceraldehyde-3-phosphate dehydrogenase at pH 6.1? [Dropdown for selection]

The isoelectric point of glutamic acid is 3.22; asparagine is 5.41.

- During paper electrophoresis at pH 6, toward which electrode does glutamic acid migrate? [Dropdown for selection]
- During paper electrophoresis at pH 6.5, toward which electrode does asparagine migrate? [Dropdown for selection]

The text requires an understanding of the relationship between pH and isoelectric point to determine the net charge and migration direction during electrophoresis.

Expert Solution

Step 1: The net charge on a protein and isoelectric point

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The isoelectric point (pl) for a buffer is identified as the pH value at which there is no net charge present on the protein. When the pH of the solution is greater than the pl value, the net charge carried by the protein will be negative and when the pH of the solution is less than the pl value, the net charge carried by the protein will be positive.

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