4. Suppose the data shown below are obtained for an enzyme catalyzed reaction. [S] (MM) v (mmol/ml/min) 0.1 3.33 0.2 0.5 0.8 1.0 2.0 5.0 7.14 8.0 8.3 9.09 a) Plot the data and determine Km and Vmax (please include the plot). b) Assuming the enzyme was used in these reactions at a concentration of 106 M, then calculate the turnover number for the enzyme.

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4. Suppose the data shown below are obtained for an enzyme catalyzed reaction.
[S] (MM)
v (mmol/ml/min)
0.1
3.33
0.2
5.0
0.5
7.14
0.8
8.0
1.0
8.3
2.0
9.09
a) Plot the data and determine Km and Vmax (please include the plot).
b) Assuming the enzyme was used in these reactions at a concentration of 10-6 M, then calculate the
turnover number for the enzyme.
Transcribed Image Text:4. Suppose the data shown below are obtained for an enzyme catalyzed reaction. [S] (MM) v (mmol/ml/min) 0.1 3.33 0.2 5.0 0.5 7.14 0.8 8.0 1.0 8.3 2.0 9.09 a) Plot the data and determine Km and Vmax (please include the plot). b) Assuming the enzyme was used in these reactions at a concentration of 10-6 M, then calculate the turnover number for the enzyme.
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