What mutant of Dihydroorotase affected the most it's turnover rate? Estimate its impact on theenzyme's turnover rate.Table 3: Kinetic Parameters for Mutants of Dihydroorotasekcat (s-1)Km (mM)kca/ K„ (M¯' s-')wild typeD250A160 +8<0.01%12 +1<0.011.7 + 0.21.5 (0.2) × 10$nd6.3 (0.3) x 103ndnd4.3 (0.1) x 10!nd1.7 (0.1) x 104ndºD250ED250H1.9 + 0.3ndndº0.51 +0.1nd0.9 + 0.1D250N<0.010.022 + 0.002<0.0115 +1D250SR20QR20K

The objective of this question is to show the mutants is one that has a significant effect on…

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What mutant of Dihydroorotase affected the most it's turnover rate? Estimate its impact on the enzyme's turnover rate.

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

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Question

What mutant of Dihydroorotase affected the most it's turnover rate? Estimate its impact on the
enzyme's turnover rate.
Table 3: Kinetic Parameters for Mutants of Dihydroorotase
kcat (s-1)
Km (mM)
kca/ K„ (M¯' s-')
wild type
D250A
160 +8
<0.01%
12 +1
<0.01
1.7 + 0.2
1.5 (0.2) × 10$
nd
6.3 (0.3) x 103
nd
nd
4.3 (0.1) x 10!
nd
1.7 (0.1) x 104
ndº
D250E
D250H
1.9 + 0.3
nd
ndº
0.51 +0.1
nd
0.9 + 0.1
D250N
<0.01
0.022 + 0.002
<0.01
15 +1
D250S
R20Q
R20K

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