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What mutant of Dihydroorotase affected the most it's turnover rate? Estimate its impact on the enzyme's turnover rate.

What mutant of Dihydroorotase affected the most it's turnover rate? Estimate its impact on the enzyme's turnover rate.

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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What mutant of Dihydroorotase affected the most it's turnover rate? Estimate its impact on the enzyme's turnover rate. Table 3: Kinetic Parameters for Mutants of Dihydroorotase kcat (s-1) Km (mM) kca/ K„ (M¯' s-') wild type D250A 160 +8 <0.01% 12 +1 <0.01 1.7 + 0.2 1.5 (0.2) × 10$ nd 6.3 (0.3) x 103 nd nd 4.3 (0.1) x 10! nd 1.7 (0.1) x 104 ndº D250E D250H 1.9 + 0.3 nd ndº 0.51 +0.1 nd 0.9 + 0.1 D250N <0.01 0.022 + 0.002 <0.01 15 +1 D250S R20Q R20K
Transcribed Image Text:What mutant of Dihydroorotase affected the most it's turnover rate? Estimate its impact on the enzyme's turnover rate. Table 3: Kinetic Parameters for Mutants of Dihydroorotase kcat (s-1) Km (mM) kca/ K„ (M¯' s-') wild type D250A 160 +8 <0.01% 12 +1 <0.01 1.7 + 0.2 1.5 (0.2) × 10$ nd 6.3 (0.3) x 103 nd nd 4.3 (0.1) x 10! nd 1.7 (0.1) x 104 ndº D250E D250H 1.9 + 0.3 nd ndº 0.51 +0.1 nd 0.9 + 0.1 D250N <0.01 0.022 + 0.002 <0.01 15 +1 D250S R20Q R20K
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