Which of the following statements are correct about the process of protein structure determination by crystallography (select all that apply)? A. Purified proteins are serially diluted until there is one protein in a droplet, which promotes formation of crystals B. Robots are used to set up crystal trays in which each well cotains a different additive to see which additives cause crystal formation C. Protein crystallization enables structure determination because all the proteins in the crystal are in the same orientation. D. The primary advantage of collecting X-Ray data using a synchroton sources is the faster rate of data collection E. The phase problem in building a structural model from X-ray data can be solved by cystallizing proteins with heavy atoms such as bromine or seleno-methionine
Which of the following statements are correct about the process of protein structure determination by crystallography (select all that apply)?
A. |
Purified proteins are serially diluted until there is one protein in a droplet, which promotes formation of crystals |
|
B. |
Robots are used to set up crystal trays in which each well cotains a different additive to see which additives cause crystal formation |
|
C. |
Protein crystallization enables structure determination because all the proteins in the crystal are in the same orientation. |
|
D. |
The primary advantage of collecting X-Ray data using a synchroton sources is the faster rate of data collection |
|
E. |
The phase problem in building a structural model from X-ray data can be solved by cystallizing proteins with heavy atoms such as bromine or seleno-methionine |
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