Can someone check my answers and see if they are correct? © Macmillan Learnglutamate and lysineaspartate and valineserine and alanineglutamateaspartatealanine▼arginine and methionineglycine and leucinemethionineleucine O Macmillan LearningMixtures of amino acids can be analyzed by first separating the mixture into its components throughion-exchange chromatography.Amino acids placed on a cation-exchange resin containing sulfonate (-SO3) groups flow down the column at different ratesbecause of two factors that influence their movement: (1) ionic attraction between the sulfonate residues on the column andpositively charged functional groups on the amino acids, and (2) aggregation of nonpolar amino acid side chains with thehydrophobic backbone of the polystyrene resin.Note that the ionic attraction is more important than hydrophobicity for this column media.For each pair of amino acids, identify which will be eluted first from a cation-exchange column using a pH 7.0 buffer.

The stationary phase (-SO3-) is negatively charged (Cation-exchange chromatography). Since the…

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Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Can someone check my answers and see if they are correct?

© Macmillan Learn
glutamate and lysine
aspartate and valine
serine and alanine
glutamate
aspartate
alanine
▼
arginine and methionine
glycine and leucine
methionine
leucine

O Macmillan Learning
Mixtures of amino acids can be analyzed by first separating the mixture into its components through
ion-exchange chromatography.
Amino acids placed on a cation-exchange resin containing sulfonate (-SO3) groups flow down the column at different rates
because of two factors that influence their movement: (1) ionic attraction between the sulfonate residues on the column and
positively charged functional groups on the amino acids, and (2) aggregation of nonpolar amino acid side chains with the
hydrophobic backbone of the polystyrene resin.
Note that the ionic attraction is more important than hydrophobicity for this column media.
For each pair of amino acids, identify which will be eluted first from a cation-exchange column using a pH 7.0 buffer.

Expert Solution

Step 1

The stationary phase (-SO₃^-) is negatively charged (Cation-exchange chromatography).

Since the stationary phase (-SO₃^-) is negatively charged, an amino acid with a higher negative change will be repelled by the negatively charged stationary phase causing fast movement.

Hence an amino acid with a negative change or neutral charge will be eluted first.

Hence the order of elution time is:

Negative charge (Eluted first) < Neutral charge < Positive charge (eluted last)

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