Of the following methods listed below, which could not be used to obtain a reasonable estimate c the molecular mass of a monomeric protein? Select all that apply. Electrospray ionization mass spectrometrylsoelectric focusing O Gel filtration O All of these methods could be used O Isoelectric focusing O Sodium dodecyl sulfate polyacrylamide gel electrophoresis O Total amino acid sequence determination by Edman degradation

Of the following methods listed below, which could not be used to obtain a reasonable estimate c the molecular mass of a monomeric protein? Select all that apply. Electrospray ionization mass spectrometrylsoelectric focusing O Gel filtration O All of these methods could be used O Isoelectric focusing O Sodium dodecyl sulfate polyacrylamide gel electrophoresis O Total amino acid sequence determination by Edman degradation

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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please solve question 10
The isoelectric point (pl) can best be calculated by: Averaging the sums of all pkas. Averaging the sum of the two pKas closest to physiological pH. Averaging the two pKas "sandwiching" the zwitterionic form. Averaging the two pKas of the alpha-amino and alpha-carboxy groups.
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1 Submit Q10. !! Unanswered Q10. An average amino acid is about 110 Da (Daltons). If there are 1273 amino acids in the SARS-CoV-2 spike protein, what do you expect the mass of spike to be? How does that compare to the expected mass of about 200 KDa? more than expected by about 90 Da Less than expected by about 90 Da B. more than expected by by about 60 KDa less than expected by about 60 KDa Submit odf 0/11 answered
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Hoping for solutions since I’m having a hard time with this. Pls skip if unsure or not willing to answer the subitems (these are all connected for one item). Thanks in advanced. Kr‎iel just dis‎covered a new p‎rotein, Kriel‎ase an‎d for it no‎t to unf‎old and lo‎se its fu‎nction, he realiz‎ed that the p‎rotein needs to be consta‎ntly imm‎ersed in a pH = 6.50 solut‎ion. He is the‎refore inte‎rested in pr‎eparing a 50 mL 0.08 M pH = 4.50 buff‎er to con‎tain the protein. He lis‎ted down the pu‎re acid‎s availabl‎e to his dis‎posal, and also looke‎d for their acid dissociation constant (Ka) values. Acid Name Ka1 Ka2 Ka3 Nitrous Acid (HNO2) 4.00 x 10-4 N/A N/A Acetic Acid (CH3COOH) 1.76 x 10-5 N/A N/A Ascorbic Acid (HAsc) 7.90 x 10-5 1.60 x 10-12 N/A Phosphoric Acid (H3PO4) 7.52 x 10-3 6.23 x 10-8 4.28 x 10-13 A. Iden‎tify which w‎eak acid sys‎tem is best to use in p‎reparing the buffer? Why? B. Ide‎ntify the spe‎cies which will ma‎ke up the bu‎ffer system?…
Question 1
Mass spectrometry X-ray diffraction Both techniques Answer Bank protein may be denatured small amounts of protein are sufficient can detect a covalent modification to a protein requires a large amount of protein analysis requires information about the protein-coding gene or genes protein sample must be pure protein must be conformationally stable Mass Spectrometry and x-ray diffraction are common biochemical techniques for characterizing proteins. Classify each statement based on whether it applies to MS, X-Ray, or both.
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0.9 The enzyme from the previous problem was further purified from the supernatant, after which a (Bradford) protein assay using coomassie blue was performed. The following standard curve was established using known concentrations of the enzyme. 595 0.8 0.7 0.6 0.5 80.4 0.3 0.2 0.1 y=0.5617x+0.0119 R²=0.9878 0 0.5 Protein Concentration (mg/mL) A. At what wavelength is the sample measured at? 1.5 B. Write out the equation relating OD595 and protein concentration. C. After purification, the protein sample was diluted 10-fold and then reacted with Coomassie Blue that resulted in an OD595 of 0.7. What was the concentration of protein in the supernatant? D. The purified protein was concentrated to a final volume of 1 L. What was the final amount of purified protein recovered? 200 g of the hulls initially at 25°C was placed into a perfectly insulated calorimeter with 500 g of isopropyl alcohol (IPA) at 5°C without stirring. At equilibrium, the temperature of the IPA was found to be 13.7°C. =…