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1) You are asked to prepare a buffer for a protein assay. This protein works best at a slightly acidic pH  of 5.5.   a. From the list of Acids and conjugate bases which pair would you pick.  (The list is in the attached image)    b. You are asked to make a 1M stock solu on of the Buffer, how many grams of each acid  and base would you need to make a 500mL stock. c. For the experiment, the final concentra on of the buffer should be 50mM. How many mL  of your stock would you need to make 2L. Hint: M1V1=M2V2

1) You are asked to prepare a buffer for a protein assay. This protein works best at a slightly acidic pH  of 5.5.   a. From the list of Acids and conjugate bases which pair would you pick.  (The list is in the attached image)    b. You are asked to make a 1M stock solu on of the Buffer, how many grams of each acid  and base would you need to make a 500mL stock. c. For the experiment, the final concentra on of the buffer should be 50mM. How many mL  of your stock would you need to make 2L. Hint: M1V1=M2V2

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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1) You are asked to prepare a buffer for a protein assay. This protein works best at a slightly acidic pH 
of 5.5.  
a. From the list of Acids and conjugate bases which pair would you pick.  (The list is in the attached image)
  
b. You are asked to make a 1M stock solu on of the Buffer, how many grams of each acid 
and base would you need to make a 500mL stock.

c. For the experiment, the final concentra on of the buffer should be 50mM. How many mL 
of your stock would you need to make 2L. Hint: M1V1=M2V2 

Table 10.2 continued Acid name acetic acid dihydrogen citrate ion butanoic aicd propanoic acid pyridinium ion carbonic acid hydrogen citrate ion hydrogen sulfite ion phenol hydrogen carbonate ion Acid formula diethylammonium ion methylammonium ion propylammonium ion dimethylammonium ion ethylammonium ion triethylammonium ion hydrogen phosphate ion CH3COOH C₂H₂O(COOH)₂COo- C₂H₂COOH C₂H5COOH CH,NH+ HOCOOH dihydrogen phosphate ion H₂PO hypochlorous acid HCIO ammonium ion hydrocyanic acid C₂H₂O(COOH)(COO)₂ HSO3 [HOSOO-] NH HCN C6H5OH HOCOO- (C,Hs),NH; CH3NH C3H₂NH3 (CH3)₂NH2 C,H,NH, (C₂H5)3NH+ HPO- pK₂ Conjugate base Conjugate base name 4.75 CH3COO- acetate ion 4.77 C3H₂O(COOH) (COO)₂ hydrogen citrate ion 4.82 C₂H₂COO- butanoate ion 4.87 C₂H5COO™ 5.25 C₂H₂N 6.37 HOCOO™ 6.40 C3H₂O(COO-) 3 7.19 SO3- 7.20 HPO- 7.53 CIO- 9.25 9.31 10.00 C6H5O 10.25 CO²- 10.49 | (C,Hs),NH 10.64 CH3NH₂ NH3 CN- 10.71 | C3H,NH, 10.77 (CH3)2NH 10.87 | C,H,NH, 11.01 (C₂H5)3N 12.38 PO3- propanoate ion pyridine hydrogen carbonate ion citrate ion sulfite ion hydrogen phosphate ion hypochlorite ion ammonia cyanide ion phenolate ion carbonate ion diethylamine methylamine propylamine dimethylamine ethylamine triethylamine phosphate ion For the negative ions please use the Na salt. For the positive ions use the Cl salt
Transcribed Image Text:Table 10.2 continued Acid name acetic acid dihydrogen citrate ion butanoic aicd propanoic acid pyridinium ion carbonic acid hydrogen citrate ion hydrogen sulfite ion phenol hydrogen carbonate ion Acid formula diethylammonium ion methylammonium ion propylammonium ion dimethylammonium ion ethylammonium ion triethylammonium ion hydrogen phosphate ion CH3COOH C₂H₂O(COOH)₂COo- C₂H₂COOH C₂H5COOH CH,NH+ HOCOOH dihydrogen phosphate ion H₂PO hypochlorous acid HCIO ammonium ion hydrocyanic acid C₂H₂O(COOH)(COO)₂ HSO3 [HOSOO-] NH HCN C6H5OH HOCOO- (C,Hs),NH; CH3NH C3H₂NH3 (CH3)₂NH2 C,H,NH, (C₂H5)3NH+ HPO- pK₂ Conjugate base Conjugate base name 4.75 CH3COO- acetate ion 4.77 C3H₂O(COOH) (COO)₂ hydrogen citrate ion 4.82 C₂H₂COO- butanoate ion 4.87 C₂H5COO™ 5.25 C₂H₂N 6.37 HOCOO™ 6.40 C3H₂O(COO-) 3 7.19 SO3- 7.20 HPO- 7.53 CIO- 9.25 9.31 10.00 C6H5O 10.25 CO²- 10.49 | (C,Hs),NH 10.64 CH3NH₂ NH3 CN- 10.71 | C3H,NH, 10.77 (CH3)2NH 10.87 | C,H,NH, 11.01 (C₂H5)3N 12.38 PO3- propanoate ion pyridine hydrogen carbonate ion citrate ion sulfite ion hydrogen phosphate ion hypochlorite ion ammonia cyanide ion phenolate ion carbonate ion diethylamine methylamine propylamine dimethylamine ethylamine triethylamine phosphate ion For the negative ions please use the Na salt. For the positive ions use the Cl salt
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