Many of the Western blot immunodetection buffers are based on a buffer solution abbreviated TBS-T. Tris Buffered Saline-Tween (TBS-T) 50 mM Tris-Cl, pH 7.6 150 mM NaCl 0.1% Tween-20 note that Tris and NaCl are crystals but Tween-20 is a detergent (similar to SDS but neutral) that is bought as a concentrate of 10% and will foam if stirred excessively We will need about 700 ml of TBS-T overall. Write out exactly how you would make this solution.
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Many of the Western blot immunodetection buffers are based on a buffer solution abbreviated TBS-T.
Tris Buffered Saline-Tween (TBS-T)
- 50 mM Tris-Cl, pH 7.6
- 150 mM NaCl
- 0.1% Tween-20
note that Tris and NaCl are crystals but Tween-20 is a detergent (similar to SDS but neutral) that is bought as a concentrate of 10% and will foam if stirred excessively
We will need about 700 ml of TBS-T overall. Write out exactly how you would make this solution.
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