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Agarose gel electrophoresis is relatively simple and straight forward to perform. Agarose is a natural linear polysaccharide of galactose and 3,6-anhydrogalactose derived from the agar (an algae) of Gelidium amansii. When dry agarose is dissolved in boiling water, poured into a mold and allowed to cool, gel formation occurs at about 45°C. The fibers soak up water and form a network of double strands held together by hydrogen bonding and hydrophobic interactions. The resulting gel is highly porous with little cross-linking among the linear polymer chains. Pore size in the agarose gel depends on agarose concentration; the higher the concentration, the smaller the pore size. At usual agarose concentrations of 0.4 to 2% (w/v), the pores are large enough to allow even the largest protein molecules to pass unimpeded. Therefore, agarose gel electrophoresis separates charged particles primarily according to charge. Particles with larger charge-to-mass ratio will be propelled through the gel by the electric field at higher velocity. Two repeating units of agarose are shown below. OH OH OH OH CH₂ H H t OH H OH OH On a piece of paper, draw chemical structures (Haworth projections) of galactose and 3,6- anhydrogalactose, the two monomers present in the natural polysaccharide, agarose. Label each with their names and upload your answer.

Agarose gel electrophoresis is relatively simple and straight forward to perform. Agarose is a natural linear polysaccharide of galactose and 3,6-anhydrogalactose derived from the agar (an algae) of Gelidium amansii. When dry agarose is dissolved in boiling water, poured into a mold and allowed to cool, gel formation occurs at about 45°C. The fibers soak up water and form a network of double strands held together by hydrogen bonding and hydrophobic interactions. The resulting gel is highly porous with little cross-linking among the linear polymer chains. Pore size in the agarose gel depends on agarose concentration; the higher the concentration, the smaller the pore size. At usual agarose concentrations of 0.4 to 2% (w/v), the pores are large enough to allow even the largest protein molecules to pass unimpeded. Therefore, agarose gel electrophoresis separates charged particles primarily according to charge. Particles with larger charge-to-mass ratio will be propelled through the gel by the electric field at higher velocity. Two repeating units of agarose are shown below. OH OH OH OH CH₂ H H t OH H OH OH On a piece of paper, draw chemical structures (Haworth projections) of galactose and 3,6- anhydrogalactose, the two monomers present in the natural polysaccharide, agarose. Label each with their names and upload your answer.

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Agarose gel electrophoresis is relatively simple and straight forward to perform. Agarose is a natural linear polysaccharide of galactose and 3,6-anhydrogalactose derived from the agar (an algae) of Gelidium amansii. When dry agarose is dissolved in boiling water, poured into a mold and allowed to cool, gel formation occurs at about 45°C. The fibers soak up water and form a network of double strands held together by hydrogen bonding and hydrophobic interactions. The resulting gel is highly porous with little cross-linking among the linear polymer chains. Pore size in the agarose gel depends on agarose concentration; the higher the concentration, the smaller the pore size. At usual agarose concentrations of 0.4 to 2% (w/v), the pores are large enough to allow even the largest protein molecules to pass unimpeded. Therefore, agarose gel electrophoresis separates charged particles primarily according to charge. Particles with larger. charge-to-mass ratio will be propelled through the gel by the electric field at higher velocity. Two repeating units of agarose are shown below. OH OH OH CH₂ H H Ĥ 01 4 H 1 ma OH OH OH On a piece of paper, draw chemical structures (Haworth projections) of galactose and 3,6- anhydrogalactose, the two monomers present in the natural polysaccharide, agarose. Label each with their names and upload your answer. OH H
Transcribed Image Text:Agarose gel electrophoresis is relatively simple and straight forward to perform. Agarose is a natural linear polysaccharide of galactose and 3,6-anhydrogalactose derived from the agar (an algae) of Gelidium amansii. When dry agarose is dissolved in boiling water, poured into a mold and allowed to cool, gel formation occurs at about 45°C. The fibers soak up water and form a network of double strands held together by hydrogen bonding and hydrophobic interactions. The resulting gel is highly porous with little cross-linking among the linear polymer chains. Pore size in the agarose gel depends on agarose concentration; the higher the concentration, the smaller the pore size. At usual agarose concentrations of 0.4 to 2% (w/v), the pores are large enough to allow even the largest protein molecules to pass unimpeded. Therefore, agarose gel electrophoresis separates charged particles primarily according to charge. Particles with larger. charge-to-mass ratio will be propelled through the gel by the electric field at higher velocity. Two repeating units of agarose are shown below. OH OH OH CH₂ H H Ĥ 01 4 H 1 ma OH OH OH On a piece of paper, draw chemical structures (Haworth projections) of galactose and 3,6- anhydrogalactose, the two monomers present in the natural polysaccharide, agarose. Label each with their names and upload your answer. OH H
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