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Mass spectroscopy Amino Acid Molecular Weight Alanine 89 Arginine 174 n Aspartic acid 133 t Glutamic acid 146 Glycine 75 Lysine 146 OES e 137 194 425 n 105 384 619 Proline 115 146 340 473 Serine 105 t 89 279 530 Tyrosine 181 Valine 117 100 200 300 400 + 500 600 Mass/charge (here the charge is -1, so it's just mass) Above is shown mass spectroscopy for a peptide 5 amino acids long derived from a soluble protein. This 5 amino acid peptide represents a hydrophilic surface part of the protein (this is a hint as to the composition of this peptide). Determine and give the amino acid sequence of this pentapeptide. Show which end is the amino terminus and which is the carboxy terminus. NOTE: (1) for simplicities sake not all possible peaks are shown, though all relevant peaks are. (2) Not all amino acids are listed, but all relevant/necessary amino acids are. (3) In reality, the masses given should be less by one (loss of H ion to impose a charge on the molecules).

Mass spectroscopy Amino Acid Molecular Weight Alanine 89 Arginine 174 n Aspartic acid 133 t Glutamic acid 146 Glycine 75 Lysine 146 OES e 137 194 425 n 105 384 619 Proline 115 146 340 473 Serine 105 t 89 279 530 Tyrosine 181 Valine 117 100 200 300 400 + 500 600 Mass/charge (here the charge is -1, so it's just mass) Above is shown mass spectroscopy for a peptide 5 amino acids long derived from a soluble protein. This 5 amino acid peptide represents a hydrophilic surface part of the protein (this is a hint as to the composition of this peptide). Determine and give the amino acid sequence of this pentapeptide. Show which end is the amino terminus and which is the carboxy terminus. NOTE: (1) for simplicities sake not all possible peaks are shown, though all relevant peaks are. (2) Not all amino acids are listed, but all relevant/necessary amino acids are. (3) In reality, the masses given should be less by one (loss of H ion to impose a charge on the molecules).

Biology 2e
Biology 2e
2nd Edition
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark
Chapter3: Biological Macromolecules
Section: Chapter Questions
Problem 23CTQ: Amino acids have the generic structure seen below, where R represents different carbon-based side...
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Please help with this Mass Spectrometry Question. Thank you

For the mass spec. shown in the attached image, please determine and give the amino acid sequence of the pentapeptide. Show which end is the amino terminus and which is the carboxy terminus. How does one arrive at the solution?

Mass spectroscopy Amino Acid Molecular Weight Alanine 89 Arginine 174 n Aspartic acid 133 t Glutamic acid 146 Glycine 75 Lysine 146 OES e 137 194 425 n 105 384 619 Proline 115 146 340 473 Serine 105 t 89 279 530 Tyrosine 181 Valine 117 100 200 300 400 + 500 600 Mass/charge (here the charge is -1, so it's just mass) Above is shown mass spectroscopy for a peptide 5 amino acids long derived from a soluble protein. This 5 amino acid peptide represents a hydrophilic surface part of the protein (this is a hint as to the composition of this peptide). Determine and give the amino acid sequence of this pentapeptide. Show which end is the amino terminus and which is the carboxy terminus. NOTE: (1) for simplicities sake not all possible peaks are shown, though all relevant peaks are. (2) Not all amino acids are listed, but all relevant/necessary amino acids are. (3) In reality, the masses given should be less by one (loss of H ion to impose a charge on the molecules).
Transcribed Image Text:Mass spectroscopy Amino Acid Molecular Weight Alanine 89 Arginine 174 n Aspartic acid 133 t Glutamic acid 146 Glycine 75 Lysine 146 OES e 137 194 425 n 105 384 619 Proline 115 146 340 473 Serine 105 t 89 279 530 Tyrosine 181 Valine 117 100 200 300 400 + 500 600 Mass/charge (here the charge is -1, so it's just mass) Above is shown mass spectroscopy for a peptide 5 amino acids long derived from a soluble protein. This 5 amino acid peptide represents a hydrophilic surface part of the protein (this is a hint as to the composition of this peptide). Determine and give the amino acid sequence of this pentapeptide. Show which end is the amino terminus and which is the carboxy terminus. NOTE: (1) for simplicities sake not all possible peaks are shown, though all relevant peaks are. (2) Not all amino acids are listed, but all relevant/necessary amino acids are. (3) In reality, the masses given should be less by one (loss of H ion to impose a charge on the molecules).
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