lon1. Below is a pedigree of a rare trait that is associated with a variable numberrepeat. PCR was performed on individuals using primers flanking the VNR,and results are shown on the agarose gel below the pedigree.I.1 1.2 II.1 II.2 II.3 II.4 II.5 II.6 11.7 III.1 III.2 III.3 III.4etumA. (5 points) What is the mode of inheritance?B. (10 points) Fill in the expected gel lanes for II.1, II.5, III.2, III.3 and III.4C. (5 points) How might you explain the gel results for II.4?

A. Mode of InheritanceThe trait exhibits an autosomal dominant mode of inheritance, as the affected…

Answered: lon 1. Below is a pedigree of a rare…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Below are several problems frequently faced by researchers when running the PCR. Give one (1) solution to each problem stated. 1. No PCR product 2. Multiple bands appeared after gel electrophoresis (you are amplifying just 1 gene) 3. Bright thick bands at the end of the agarose gel after electrophoresis 4. Bands appeared at the negative control
If possible please answer all questions based on the gel electrophoresis: 1. What is PCR product size?2. Which reactions were successful/unsuccessful? Which reactions showed clean products? (J1...J6 = jump lines), (N= negative control) & (P= positive control)
What is the final volume of the individual PCR reactions we are making?
1. Explain what primers are and what purpose they serve in a PCR reaction. Explain the main steps and temperatures of one PCR cycle. 2. Explain the purposes of the following components of gel electrophoresis: agarose, loading buffer, DNA ladder/marker, electrode buffer, electrodes. 3. Explain the expected results of the Alu sequence at PV92 that we tested, in terms of numbers of bands and their sizes. A labeled drawing would suffice.
Part A. If student counts 63 colonies on their 10^-6 dilution LB plate. What was the original concentration of their cells if they plated 100ul? Part B.If we used pGFPuv as the template for PCR positive control. This is because: a. it contains the GFP gene so it should show a product. b. It contains DNA fragments that were added to the ligation reaction. c. It is the desired plasmid we wanted to make. d. So we have a band to compare our unknown plasid to allowing us to check if the unknown is the right size.
at Arrange Window Help 4) 57% D Tue 4 May Tools Slide Show 2 ARMS PCR nations Slide Show Review View A A =、=<|EE|三v| v v A Sha v 20 Quick Styles D Sha Text Arrange Convert to SmartArt Picture Shapes ab x x. AV v Aa v D. Av A、申。 Box Briefly outline how MLPA probe-sets can simultaneously detect multiple targets using a single set of PCR primers. E Notes Comments MAY 4 Pr w MacBook Air
(a) To illustrate how the ADP phosphorylative capacity was measured, the diagram below on the left shows a ³1P NMR spectrum of the arm of a control female subject at rest, and the diagram on the right the changes measured in the creatine and phosphocreatine levels of the arm at rest and exercise. PCr 10 Exer -cise Exer →→→Rest◄cise Rest PCr/Pi 9 PCr 15 20 10 Time(min) Isokinetic ergometer exercise of the control female subject showing intervals of rest and exercise. To obtain this graph, a ³1P NMR spectrum was col- lected every 2 min to show the changes in the PCr and Pi content of the arm skeletal muscle tissue. NH₂ during activity + ATP + C=NH2 during recovery YATP Pi GATP BATP SP 15 0 10 15 20 25 5 ppm 31P NMR spectrum of the arm of a control sub- ject at rest showing characteristic resonance features for the phosphate residues of ATP, phosphocreatine (PCr), and Pi. At rest the PCr/Pi ratio is ordinarily ~ 8-9. The synthesis of phosphocreatine is unfavorable. If dur- ing extended…
Run a barcoding protocol with rbcL gene-specific primers. Use 30 cycles for your PCR amplification profile. Complete the table below for your PCR cocktail.
a. Draw a diagram of what occurs in PCR during the annealing step of the FIRST cycle. b. Draw a diagram of what occurs in the annealing step in the LAST cycle in the vast majority of cases.
Complete the table. Answer the red colors.
In one PCR cycle, the correct order of steps isa. primer annealing, primer extension, denaturation.b. primer annealing, denaturation, primer extension.c. denaturation, primer annealing, primer extension.d. denaturation, primer extension, primer annealing.
Answer each of the following correctly. Designer Genes Work (This is all about Applications of Recombinant DNA) 1. How does DNA Replicate? 2. What is Genetically Modified Organism (GMO)? 3. Illustrate your own Designer genes based on the following: 1. Identify a special trait. 2. Identify a source organism. 3. Identify a target orgsnism 4. Identify the modified/added trait.

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