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| a) What is the negative control? |
| b)Why are two controls used? |
| c) Why incubate the plate? |
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- a) Which sample(s) contained the antigen? b)The samples you added to the microplate strip contain many proteins and may or may not contain the antigen. What happened to the proteins in the plastic well if the sample contained the antigen? If it did not contain the antigen? c) Why did you need to wash the wells after every step?-For each item listed below, select a sterilant that you feel is the most suitable and effective for that item. Discuss why you selected each sterilant.? A) plastic Petri dishes to be used for nutrient media B) glass tubes of bacterial cultures to be discarded C) sterile milk D) dozens of reusable glass pipets E) bone tissue for grafting F) room air G) hospital mattresses H) biopsy tissue for histology analysis I) plastic respiratory therapy equipment and arthroscopes J) vaccinesWhat method do ELISA’s utilize in their measurement? a) light absorption (eg Abs480nm) b) b+–radiation c) combination of absorption and radiation d) electrophoresis
- 23. A good blood smear should: a) Have feathered edges b) Occupies 2/3 Slide c) Fingerprint shape d) Not Contain clotted blood e) All the above 24. If a technician collects 1 ml blood in 2.5 ml light blue tube: a) Reject the sample and cancel the test b) No problem, this is normal ratio c) Have the sample re-taken d) Consult your supervisor e) Report to physician 25. Thin blood smear remedy action is: a) Increase the angle of the spreader and bigger drop of blood b) Decrease the angle of spreader and bigger drop of blood c) Increase the angle of spreader and smaller drop of blood d) Decrease the angle of spreader and smaller drop of blood 26. The centrifuge used in hematocrit test is: a) Centrifuge b) Cytospin c) Microcentirfuge d) Microhematocrit 27. The sediment observed in stained blood smear caused by: a) Overstraining b) More Haematoxylin in the mixture c) Smear was not fixed d) Using non- filtered stain e) Using old stain 28. Anticoagulant present in Lavender tube is a) Sodium…6) protein denaturation caused by concentration of salts and minerals in solution is a) coagulation b) Retrogradation c) Salting in d) non of the mentioned e) Hardening-Two hundred patients without chest pain were screened with a new biochemical test that showed 190 had a negative screening result. What is the test's specificity? a) 80% b) 95% c) 90% d) 75%
- In a direct ELISA, which of the following does the patient provide? A.) Substrate B.) Enzymes C.) Antibodies D.) Antigens5) A pure culture A) is sterile . B) is a population of identical cells. C) is made of a clearly defined chemical medium. D) contains one microbial cell.21) Define the term antibiotic 22) What is the name of the circle formed around each of the discs above? 23-25) The antibiotics should be classified as R, I, or S. What do these initials stand for?
- 20) which of the following types of information is provided by a suffix? A) numbers b) positions c) times d) diagnostic proceduresa)At what wavelength (nm) on a spectrometer can this color be quantified? b) Record your results. Indicate on this page which boxes turned color. A B C + - 1:2 1:10 1:100 c) Based on these results, which patient(s) definitely have lupus, which definitely do not, and which require further testing?Refer to the figures above: 41) Which figure displays the rate of bacterial growth in a living animal? a) Figure A b) Figure C C) Both d) Neither e) Cannot be determined 42) Which figure tests the effects of one or more lymphocytes? a) Figure A b) Figure C c) Both d) Neither e) Cannot be determined 43) Which figure demonstrates that addition of effector PCs leads to bacterial death? a) Figure A b) Figure C C) Both d) Neither e) Cannot be determined 44) Based on these figures, Listeria infection control is most directly due to which effector cell: a) CTL b) T helper c) Macrophage d) Basophil d) None of the above