= Menu #2 Mol Bio Mutation QUESTION... # 3 Mol Bio Gene of inter.. X + Create All tools Edit Convert E-Sign 9 Sign in Find text or tools H Q 2 You discovered that a species of bacteria can break down Styrofoam™ (polystyrene) products due to an enzyme it produces, polystyrenase. You wish to study the gene that codes for this enzyme. Tack 1: DNA Extraction To begin work on the bacterium, you begin by extracting its genomic DNA (gDNA). What is the purpose of the following procedures? Answer briefly but completely. a. Using sodium dodecyl sulfate, a detergent Answer: b. Adding RNase A and Proteinase K during extraction Answer: ☑ 0 Al Assistant Task 2: Polymerase Chain Reaction After purifying the gDNA extract, you want to isolate and amplify the polystyrenase gene. You perform PCR using the appropriate gene-targeted primers. What is the purpose of the following PCR components? Answer briefly but completely. a. DNA polymerase isolated from Thermus aquaticus Answer: Dr C. Adding ethanol before recovering the DNA extract Answer: b. Deoxynucleotide triphosphates (dNTPs) Answer: C. Forward and reverse primers Answer: Q Search Task 3: Agarose Gel Electrophoresis Your amplicon from PCR was subjected to AGE for analysis. You are shown the image of the gel loaded with the following lanes: (A) negative control, (B) size ladder (1, 2, 3, 4, 6, and 10 kb), (C) gDNA extract, (D) PCR amplicon. However, due to mishaps while loading the gel with the samples, you are not sure which lane is which. You are shown a diagram of the obtained gel below. IA ༢ ☑ 1 2 C 1:1 9:14 PM 10/23/2024 Q = Menu #2 Mol Bio Mutation QUESTION... # 3 Mol Bio Gene of inter.. X + Create All tools Edit Convert E-Sign 9 Q Search Sign in Find text or tools H a. Label each lane of the gel. Write only the corresponding letters in the wells above. b. Above each band in the size ladder, write its size (in kb). Approximate the size (in kb) of the polystyrenase gene. Write your answer above the band corresponding to the gene. Bonus: If you wish to identify the bacterial species in this scenario, what gene is most commonly and routinely sequenced? Answer: 画 IA W ☑ Σ 0 Al Assistant ༢ ☑ C 2 2 с 9:14 PM Q 10/23/2024

= Menu #2 Mol Bio Mutation QUESTION... # 3 Mol Bio Gene of inter.. X + Create All tools Edit Convert E-Sign 9 Sign in Find text or tools H Q 2 You discovered that a species of bacteria can break down Styrofoam™ (polystyrene) products due to an enzyme it produces, polystyrenase. You wish to study the gene that codes for this enzyme. Tack 1: DNA Extraction To begin work on the bacterium, you begin by extracting its genomic DNA (gDNA). What is the purpose of the following procedures? Answer briefly but completely. a. Using sodium dodecyl sulfate, a detergent Answer: b. Adding RNase A and Proteinase K during extraction Answer: ☑ 0 Al Assistant Task 2: Polymerase Chain Reaction After purifying the gDNA extract, you want to isolate and amplify the polystyrenase gene. You perform PCR using the appropriate gene-targeted primers. What is the purpose of the following PCR components? Answer briefly but completely. a. DNA polymerase isolated from Thermus aquaticus Answer: Dr C. Adding ethanol before recovering the DNA extract Answer: b. Deoxynucleotide triphosphates (dNTPs) Answer: C. Forward and reverse primers Answer: Q Search Task 3: Agarose Gel Electrophoresis Your amplicon from PCR was subjected to AGE for analysis. You are shown the image of the gel loaded with the following lanes: (A) negative control, (B) size ladder (1, 2, 3, 4, 6, and 10 kb), (C) gDNA extract, (D) PCR amplicon. However, due to mishaps while loading the gel with the samples, you are not sure which lane is which. You are shown a diagram of the obtained gel below. IA ༢ ☑ 1 2 C 1:1 9:14 PM 10/23/2024 Q = Menu #2 Mol Bio Mutation QUESTION... # 3 Mol Bio Gene of inter.. X + Create All tools Edit Convert E-Sign 9 Q Search Sign in Find text or tools H a. Label each lane of the gel. Write only the corresponding letters in the wells above. b. Above each band in the size ladder, write its size (in kb). Approximate the size (in kb) of the polystyrenase gene. Write your answer above the band corresponding to the gene. Bonus: If you wish to identify the bacterial species in this scenario, what gene is most commonly and routinely sequenced? Answer: 画 IA W ☑ Σ 0 Al Assistant ༢ ☑ C 2 2 с 9:14 PM Q 10/23/2024

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter16: Reproductive Technology, Genetic Testing, And Gene Therapy

Section: Chapter Questions

Problem 13QP: The prospect of using gene therapy to alleviate genetic conditions is still a vision of the future....

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