Not use ai please The production of large number of identical copies of gene-sized pieces of DNA definessplicing.recombinant DNA.O cloning.transformation.

Cloning refers to the process of producing a large number of identical copies of a gene-sized piece…

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Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter15: Genomes And Genomics

Section: Chapter Questions

Problem 6QP: Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel,...

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What is the function of DNA polymerase? a. It degrades DNA in cells. b. It adds RNA nucleotides to a new strand. c. It coils DNA around histones to form chromosomes. d. It adds DNA nucleotides to a replicating strand. e. None of these.
Cloning Genes Is a Multistep Process Which enzyme is responsible for covalently linking DNA strands together? a. DNA polymerase b. DNA ligase c. EcoRl d. restriction enzymes e. RNA polymerase
How does DNA replication occur in a precise manner to ensure that identical genetic information is put into the new chromatid? See Figures 8.12 and 8.13. FIGURE 8.12 In DNA replication, the two polynucleotide strands uncoil, and each is a template for synthesizing a new strand. A replicated DNA molecule contains one new strand and one old strand. This mechanism is called semiconservative replication. FIGURE 8.13 A close-up look at the process of DNA replication. (a) As the strands uncoil, bases are added to the newly synthesized strand by complementary base pairing with bases in the template strand. The new bases are linked together by DNA polymerase. (b) DNA synthesis can proceed only in the 5 3 direction; newly synthesized DNA on one template strand is made in short segments and linked together by the enzyme DNA ligase.
generated after a cDNA library is made unique sequences in the genome useful for mapping using sequence information all of the above
What is the purpose and benefit of the polymerase chain reaction?
DNA replicates by which of the following models? a. conservative b. semiconservative c. dispersive d. none of the above
Which of the following statements is not true about DNA replication? a. It occurs during the M phase of the cell cycle. b. It makes a sister chromatid. c. It denatures DNA strands. d. It occurs semiconservatively. e. It follows base-pairing rules.
Figure 17.7 You are working in a molecular biology lab and, unbeknownst to you, your lab partner left the foreign genomic DNA that you are planning to clone on the lab bench overnight instead of storing it in the freezer. As a result, it was degraded by nucleases, but still used in the experiment. The plasmid, on the other hand, is fine. What results would you expect from your molecular cloning experiment? There will be no colonies on the bacterial plate. There will be blue colonies only. There will be blue and white colonies. The will be white colonies only.
Cloning Genes Is a Multistep Process The following DNA sequence contains a six-base sequence that is a recognition and cutting site for a restriction enzyme. What is this sequence? Which enzyme will cut this sequence? (See Figure 13.5 for help.) 5 CCGAGGAAGCTTAC 3 3 GGCTCCTTCGAATG 5
Match the terms appropriately. ___ nucleotide ___ clone a. nitrogen-containing base, sugar, phosphate groups ___ autosome b. copy of an organism ___ DNA polymerase c. does not determine sex ___ mutation d. injects DNA ___ bacteriophage e. replication enzyme ___ semiconservative replication f. can cause cancer g. something old, something new ___ DNA ligase h. seals gaps and breaks
Our bodies are not defenseless against mutagens that alter our genomic DNA sequences. What mechanisms are used to repair DNA?
Discuss Concepts A forensic scientist obtained a small DNA sample from a crime scene. In order to examine the sample, he increased its quantity by cycling the sample through the polymerase chain reaction. He estimated that there were 50,000 copies of the DNA in his original sample. Derive a simple formula and calculate the number of copies he will have after15 cycles of the PCR.

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The production of large number of identical copies of gene-sized pieces of DNA defines splicing. recombinant DNA. O cloning. transformation.