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- Because a newly synthesized DNA molecule consists of one old strand and one new strand, DNA replication is described as recombinatory. conservative. dispersive. semiconservative. transcriptive.An viral enzyme that can be used to create complementary cDNA is DNA polymerase. reverse transcriptase. restriction endonuclease. DNA ligase. mutase.Copying errors that slip by DNA polymerase proofreading can be corrected by DNA ligase. direct repair. mismatch repair. non-homologous end joining. homologous recombination.
- Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.What is the function of DNA polymerase? a. It degrades DNA in cells. b. It adds RNA nucleotides to a new strand. c. It coils DNA around histones to form chromosomes. d. It adds DNA nucleotides to a replicating strand. e. None of these.Cloning Genes Is a Multistep Process Which enzyme is responsible for covalently linking DNA strands together? a. DNA polymerase b. DNA ligase c. EcoRl d. restriction enzymes e. RNA polymerase
- Arrange the steps of DNA replication in the order that they occur. First step Helicase unwinds the DNA double helix. Last step Answer Bank DNA polymerase synthesizes DNA. RNA primers are added. DNA ligase joins DNA fragments together. RNA primers are removed. Single-stranded DNA-binding proteins bind to each template strand.Which of the following are major differences between DNA and RNA polymerases? RNA polymerase, but not DNA polymerase, requires a primer to begin synthesizing the nucleic acid polymer DNA polymerase, but not RNA polymerase, requires a primer to begin synthesizing the nucleic acid polymer RNA polymerase, but not DNA polymerase, is unique to eukaryotic cells DNA polymerase, but not RNA polymerase, is unique to eukaryotic cells DNA polymerase is involved in replication; RNA polymerase is involved in transcription 00Which of these molecules links the most of the individual DNA nucleotides together on the newly synthesized strands of DNA? topoisomerase DNA polymerase ligase RNA primase helicase
- In which of the following processes is reverse transcriptase involved? More than one answer is correct. Options: Chromatin condensation. Transposition of a retrotransposon. Kinetochore assembly. Construction of a cDNA library. mRNA synthesis.The ADNA genetic map (Figure 1), showing the positions of the important genes and the functions of the gene clusters. Lysogeny DNA replication CI CRO CHO CII Regulator of late genes Excision/ Integration Prophage end Lysis protein JIKLMHT EFEDCBWA sVU cos Tail genes Figtre The DNA can be increased in size by only about 5%, representing the addition of only 3 kb of new DNA. You are developing a cloning vector based on i bacteriophage (Figure 1). Which segment of the genes can be removed from the DNA to increase the ability to carry a larger DNA fragment? Explain your reason. Head genes How lambda DNA exists as a double stranded linear molecule during the infection cycle? Spx su peWhich of the following has activity that is most similar to RNA Polymerase? Helicase DNA Polymerase Primase Exonuclease
