In bacterial transformation ... 1, Why do you have to incubate the competent bacterial cells on ice after the addition of plasmid DNA? 2. Why do you have to add SOC (Super Optimal Broth with Catabolite repression) medium to bacterial cells? 3. Why do you have to incubate the bacterial cells in SOC medium at 37oC for 45 minutes

Q: Kpnl 4. Plasmid Z has a size of 7 kb, and the map shows Kpnl (K) and Pstl (P) cut sites relative to…

A: Bg2(B) contians restriction site in between Pst1 and Kpn1. Given plasmid have one restriction site…

Q: Q6) Why are ‘sticky ends’ useful biotechnologically? Q7) Why is this enzyme called a restriction…

A: When restriction enzymes cut between two bases on the same strand at one end of the sequence and two…

Q: Isolation of DNA is a crucial step for genetic engineering. (i) What is the difference between…

A: INTRODUCTION Genomic DNA Genomic DNA is the genetic material of the organism that contain hereditary…

Q: Antisense therapeutics are important because: Select one: a. They only act at the ribosome O b. They…

A: Therapeutics Therapeutic is the medicinal branch dealing with the prevention and treatment of…

Q: Could you explain how you would know if you successfully induced transformation? Thank you Did you…

A: Transformation experiments are very crucial for understanding fundamental biological processes.…

Q: 24. In recombinant DNA technology, genes are usually inserted into what form of DNA before being…

A: Recombinant DNA technology is a technique for isolating segments of DNA from various species. The…

Q: A thermophillic bacterium was isolated from a hot spring at Perak to study its thermostable amylase…

A: DNA technology is the chemical manipulation of the genotype and the resulting phenotype of organisms…

Q: 3a please in simple language

A: Introduction  A important historical experiment that showed DNA replication's semi-conservative…

Q: Provide a detailed description and hand drawn figure of each of the following. (1) Mismatch…

A: Although the genetic variation is important for evolution, the survival of the individual demands…

Q: In preparation for transformation, why do you think we used calcium chloride and magnesium chloride…

A: We often use calcium chloride and magnesium chloride to make cell competent. As DNA is hydrophilic…

Q: Norflaxacin is a cidal broad spectrum antibiotic that functions by inhibiting DNA gyrase in gram +/-…

A: Norfloxacin is a broad-spectrum fluoroquinolone antibiotic, which is used to treat UTI (urinary…

Q: 93. Which is a common function for the protein product of a cancer-causing gene? a) a role in DNA…

A: Cancer-causing genes, like the name implies are genes that lead to the development of cancer. We…

Q: You generate several temperature sensitive mutant strains of bacteria. To study what genes might be…

A: Introduction :- Bacterial mutants frequently lose a growth characteristic (such as the inability to…

Q: and they/ it start(s) from the mRNA 4-Some/ one application(s) for DNA are/ is Therefore, the…

A: Single-stranded RNAs of the type known as messenger RNA, or mRNA, are used to make proteins. mRNA is…

Q: You are trying to isolate a mutant of wild-type E. coli thatrequires histidine for growth. This can…

A: E.coli. is a type of bacteria. Bacteria are unicellular and prokaryotic organisms that live in a…

Q: 4. The image below is based on the Meselson Stahl experiment where bacteria are grown in 15N and…

A: The Meselson-Stahl experiment showed that DNA replication is a semiconservative process. In this…

Q: When DNA probes are used to detect specific sequence similarities in bacterial DNA, the probe is…

A: The DNA probes can be single or double-stranded containing sequence-specific oligonucleotides.

Q: 4. (2 points) Pseudomonas aeruginosa's circular chromosome is 7.5x106 bp long and the replication…

A: To calculate the time required for Pseudomonas aeruginosa to replicate its genome, we can use the…

Q: It takes 40 minutes to completely replicate the E. coli chromosome, even in an optimally nourished…

A: Bacteria grow rapidly under the favorable environment where all the necessary conditions for its…

Q: Which of the following prokaryotic cells would probably not be “competent” at transformation? an…

A: Bacteria can take up foreign DNA in a process called transformation. Transformation is a key step in…

Q: Restriction enzymes in bacterial cytoplasm cut injected bacteriophage DNA wherever certain sequences…

A: The genes are the hereditary unit of an organism. Specific nucleotide sequence of a gene causes the…

Q: Draw a diagram showing what pGEM will look like after it has been digested with BamHI.

A: BamHI: BamHI is a restriction enzyme obtained from Bacillus amyloliquefaciens that specifically cuts…

Q: t 9:00 am, you measured your culture's sucrose levels to be 100 M and maltose levels to be at 0 M.…

A: Maltose is a disaccharide sugar and is made up of two glucose molecules while sucrose is made up of…

Q: How can you tell if you have had a successful induced transformation using: "C" = control, "T"…

A: Transformation refers to the process of introducing foreign DNA (such as plasmid DNA) into bacterial…

Q: During DNA replication, one of the new strands of DNA is synthesized continuously, while the other…

A: DNA is a molecule that has a repeating chain of identical five-carbon sugars (polymers) linked…

Q: Beadle and Tatum Experiment X rays Conidia from the cultures that fail to grow on minimal medium are…

A: Beadle and Tatum experiment... Beadle and Tatum started their experiment by creating mutants of the…

Q: 3). You mutagenize bacteria, plate them to the permissive temperature, then replica plate to the…

A: At the permissive temperature, the mutants in dnaB would likely exhibit a normal phenotype since…

Q: Provide a brief description behind your choice? Virus-mediated transfer of cellular genetic…

A: 1- A Transduction. Transduction is among the three basic mechanism of genetic exchange in Bacteria.…

Q: .Describe the method you would use to test for the uptake of a plasmid with a DNA insert

A: Introduction: The introduction of recombinant DNA into a suitable host cell is followed by the…

Q: 2. Consider the experiment conducted by Meselson and Stahl in which they used 14N and 15N in…

A: Literally, replication means the system of duplication. In molecular biology, DNA replication is the…

Q: During which step in the transformation protocol does the plasmid DNA move into the cell? O Heat…

A: During a procedure termed transformation, bacteria may pick up foreign DNA. In DNA cloning,…

Q: Categorize the following as being associated with transformation, conjugation, or transduction. Note…

A: There are three principal methods of genetic transfer between bacteria. Transformation deals with…

Q: Correct order ib which the following enzynes would operate to fix a damaged nucleotide in a human…

A: The correct order of enzymes used to fix a damaged nucleotide in a human gene is option d , i.e.,…

Q: ii. ) If the lac operon in 'a' carried a mutation in the CAP binding site that rendered it…

A: First it has to be understood that : 1) the CAP-cAMP complex acts as a glucose sensor and is only…

Q: Preparing plasmid (double-stranded, circular) DNA for sequencing involves annealing a complementary,…

A: The process of joining of single-stranded DNA or RNA by hydrogen bonds to form a double-stranded…

Q: Are both statements true? 1. Heterogeneous RNA is a term that refers to mRNA that has not been…

A: As you have posted more than one question we will solve first question for you as per the…

Q: Animals can also be genetically modified besides bacteria. As to date, transgenic cows, goats and…

A: Genetically modified animals are  genetically modified for various reasons such as drug production,…

Q: Would you expect transfer of chromosomal DNA by conjugation to be more efficient if cells were…

A: Bacterial conjugation is defined as the transfer of genetic material from one bacterial cell to…

Q: This strand is 18% A, 12% T, 36% G, and 34% C.

A: Chargaff's rules: As you mentioned, the key lies in remembering Chargaff's rules. Adenine (A) always…

Q: 1. Why were nuclei being unstained when you used eosin? 2. What is the relationship between…

A: Answer 1- Eosin is a group of fluorescent acidic compounds that reacts with and forms salts with…

Q: the following statements are correct about the repair of a Coli (select all that apply)? Strand A…

A: The DNA replication process is exceptionally accurate, but it is not completely infallible. Errors…