In a conjugation experiment, we used two strains: Hfr: streptomycin sensitive and F-: auxotrophic for isoleucine and threonine, streptomycin resistant. If we interrupt conjugation when only threonine gene has been transferred and spread this mixture on: Minimal media, Minimal media with streptomycin Minimal media with threonine and streptomycin Minimal media with isoleucine and streptomycin LB agar plates with streptomycin On which plates do we expect bacterial growth? Why?
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- ) In a conjugation experiment, we used two strains:
Hfr: streptomycin sensitive and
F-: auxotrophic for isoleucine and threonine, streptomycin resistant.
If we interrupt conjugation when only threonine gene has been transferred and spread this mixture on:
- Minimal media,
- Minimal media with streptomycin
- Minimal media with threonine and streptomycin
- Minimal media with isoleucine and streptomycin
- LB agar plates with streptomycin
On which plates do we expect bacterial growth? Why?
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