Besides agarose gel electrophoresis, there is also SDS-PAGE (sodium dodecyl sulphate- polyacrylamide gel electrophoresis) used commonly for protein separation. i) ii) Explain the principle of SDS-PAGE. In order to create a western blot, SDS-PAGE is first performed to separate the proteins. After the electrophoretic separation, the proteins are transferred to a nitrocellulose membrane prior to staining with antibodies specific for the protein understudy. Describe how the antibodies could aid in the protein detection process.

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