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From one Escherichia coli strain, five Hfr strains were isolated. The location and orientation of the origin of transfer of each Hfr strain are shown in Figure 1. want to use these five strains to map the locus responsible for thiamine synthesis, called thi. Each Hfr strain is sensitive to rifampicin (RifS) and Thi+. Conjugation experiments are performed between each of the Hfr strains and an F- RifR Thi 0 T leu 20 30 T nadD pyrC trp 40 his 50 60 70 80 cysG 90 metA 1.00 Hfr1 Hfr2 Hfr3 Hfr4 Hfr5 Figure 1: Chromosome map of Escherichia coli. Five Hfr strains (Hfr1 to Hfr5) were isolated and the location and orientation of the origin of transfer is shown by the arrows in each Hfr strain. Distances in minutes are indicated. Leu: leucine biosynthesis; nadD: NAD biosynthesis; pxrC: pyrimidine biosynthesis; trp: tryptophan biosynthesis; his: histidine biosynthesis; cysG: cysteine biosynthesis; metA: biosynthesis of methionine.

From one Escherichia coli strain, five Hfr strains were isolated. The location and orientation of the origin of transfer of each Hfr strain are shown in Figure 1. want to use these five strains to map the locus responsible for thiamine synthesis, called thi. Each Hfr strain is sensitive to rifampicin (RifS) and Thi+. Conjugation experiments are performed between each of the Hfr strains and an F- RifR Thi 0 T leu 20 30 T nadD pyrC trp 40 his 50 60 70 80 cysG 90 metA 1.00 Hfr1 Hfr2 Hfr3 Hfr4 Hfr5 Figure 1: Chromosome map of Escherichia coli. Five Hfr strains (Hfr1 to Hfr5) were isolated and the location and orientation of the origin of transfer is shown by the arrows in each Hfr strain. Distances in minutes are indicated. Leu: leucine biosynthesis; nadD: NAD biosynthesis; pxrC: pyrimidine biosynthesis; trp: tryptophan biosynthesis; his: histidine biosynthesis; cysG: cysteine biosynthesis; metA: biosynthesis of methionine.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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From one Escherichia coli strain, five Hfr strains were isolated. The location and orientation of the origin of transfer of each Hfr strain are shown in Figure 1. want to use these five strains to map the locus responsible for thiamine synthesis, called thi. Each Hfr strain is sensitive to rifampicin (RifS) and Thi+. Conjugation experiments are performed between each of the Hfr strains and an F- RifR Thi leu 10 20 T nadD pyrC trp 30 his Donor strain Hfr1 Hfr2 Hfr3 Hfr4 Hfr5 The results are shown in the following table 60 70 cysG Colonies Thi 1000 0 400 0 25 80 90 metA 1,00 Hfr1 Hfr2 Figure 1: Chromosome map of Escherichia coli. Five Hfr strains (Hfr1 to Hfr5) were isolated and the location and orientation of the origin of transfer is shown by the arrows in each Hfr strain. Distances in minutes are indicated. Hfr 3 Leu: leucine biosynthesis; nadD: NAD biosynthesis; pyrC: pyrimidine biosynthesis; trp: tryptophan biosynthesis; his: histidine biosynthesis; cysG: cysteine biosynthesis; metA biosynthesis of methionine. Hfr4 Hfr5 1) Propose a conjugation experiment (with the parental strains, the selection media used and the different experimental steps) and the method to determine the order and genetic determine the order and genetic distances between pyrC, trp, and thi
Transcribed Image Text:From one Escherichia coli strain, five Hfr strains were isolated. The location and orientation of the origin of transfer of each Hfr strain are shown in Figure 1. want to use these five strains to map the locus responsible for thiamine synthesis, called thi. Each Hfr strain is sensitive to rifampicin (RifS) and Thi+. Conjugation experiments are performed between each of the Hfr strains and an F- RifR Thi leu 10 20 T nadD pyrC trp 30 his Donor strain Hfr1 Hfr2 Hfr3 Hfr4 Hfr5 The results are shown in the following table 60 70 cysG Colonies Thi 1000 0 400 0 25 80 90 metA 1,00 Hfr1 Hfr2 Figure 1: Chromosome map of Escherichia coli. Five Hfr strains (Hfr1 to Hfr5) were isolated and the location and orientation of the origin of transfer is shown by the arrows in each Hfr strain. Distances in minutes are indicated. Hfr 3 Leu: leucine biosynthesis; nadD: NAD biosynthesis; pyrC: pyrimidine biosynthesis; trp: tryptophan biosynthesis; his: histidine biosynthesis; cysG: cysteine biosynthesis; metA biosynthesis of methionine. Hfr4 Hfr5 1) Propose a conjugation experiment (with the parental strains, the selection media used and the different experimental steps) and the method to determine the order and genetic determine the order and genetic distances between pyrC, trp, and thi
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