II. Use the eukaryotic gene DNA sequence below to answer the following questions: 1 11 21 CGACTTACTG 51 TGGACGCGCC 101 GTCATTCAGC GGCGTATAAA 61 111 71 31 GCGACGACTG TAGACTGATG AGCCTATCCA 121 81 41 ATGGCCCTGT AAGCGGTGCG ATGCAATAAA ACGCGTATCA 131 91 141 GTAGTCTGAT GCCAGTCGAC TGCATTGGAC ACCGGTTACA

Human Anatomy & Physiology (11th Edition)
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Chapter1: The Human Body: An Orientation
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**Eukaryotic Gene DNA Sequence Analysis**

**DNA Sequence:**

```
1     11    21    31    41
CGACTTATCG  GGCGGTATAAA  GCGACGACTG  TAGACTGATG  AGCCGTATCCA
51    61    71    81    91
TGGACGCGCC  ATGGCCCTGT  AAGCGGTGCG  ATGCAATAAA  ACCGGTATCA
101   111   121   131   141
GTCATTCAGC  GTAGCTTGAT  GCCAGTCGAC  TGGCATTGGAC  ACCCGGTTACA
```

**Questions & Tasks:**

a. Write the corresponding sequences, circle & label them in the sequence above:
   - **TATA box sequence:** ________________ (label as TATA)
   - **Polyadenylation signal in the corresponding mRNA:** ________________ (label as poly-A)
   - **Kozak's sequence in the corresponding mRNA:** ________________ (label as Kozak)
   - **Start (initiation) codon in the corresponding mRNA:** ________________ (label as start)
   - **Stop (termination) codon in the corresponding mRNA:** ________________ (label as stop)

b. What region of mRNA contains the open reading frame that will be translated into protein? Circle one.
   - 51-111
   - 61-69
   - 37-45
   - 71-81

c. Where is the 3' UTR? Circle one.
   - 70-111
   - 14-60
   - 73-90
   - 85-150

**Genetic Code Chart:**

A matrix showing the genetic code used to translate mRNA codons into amino acids. The chart is organized by the first, second, and third letters of the mRNA codon sequence. For example:
- UUU and UUC code for Phenylalanine (Phe)
- UGA codes for a Stop
- AUG codes for Methionine (Met), which is also the start codon

The chart is divided into sections based on the first letter (U, C, A, G), and within each section, codons are grouped by the
Transcribed Image Text:**Eukaryotic Gene DNA Sequence Analysis** **DNA Sequence:** ``` 1 11 21 31 41 CGACTTATCG GGCGGTATAAA GCGACGACTG TAGACTGATG AGCCGTATCCA 51 61 71 81 91 TGGACGCGCC ATGGCCCTGT AAGCGGTGCG ATGCAATAAA ACCGGTATCA 101 111 121 131 141 GTCATTCAGC GTAGCTTGAT GCCAGTCGAC TGGCATTGGAC ACCCGGTTACA ``` **Questions & Tasks:** a. Write the corresponding sequences, circle & label them in the sequence above: - **TATA box sequence:** ________________ (label as TATA) - **Polyadenylation signal in the corresponding mRNA:** ________________ (label as poly-A) - **Kozak's sequence in the corresponding mRNA:** ________________ (label as Kozak) - **Start (initiation) codon in the corresponding mRNA:** ________________ (label as start) - **Stop (termination) codon in the corresponding mRNA:** ________________ (label as stop) b. What region of mRNA contains the open reading frame that will be translated into protein? Circle one. - 51-111 - 61-69 - 37-45 - 71-81 c. Where is the 3' UTR? Circle one. - 70-111 - 14-60 - 73-90 - 85-150 **Genetic Code Chart:** A matrix showing the genetic code used to translate mRNA codons into amino acids. The chart is organized by the first, second, and third letters of the mRNA codon sequence. For example: - UUU and UUC code for Phenylalanine (Phe) - UGA codes for a Stop - AUG codes for Methionine (Met), which is also the start codon The chart is divided into sections based on the first letter (U, C, A, G), and within each section, codons are grouped by the
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