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- Enumerate the differences between a serological and a volumetric pipette and enumerate the uses, advantages, and limitations of a glass versus a plastic volumetric pipette.1) a)What is affinity chromatography and what is it used for? b) A drug called Bamlanivimab is used to treat infections with SARS-CoV-2 (Covid-19).This drug was developed by isolating antibodies from the serum of a patient who recovered from Covid-19.What is possibly on the stationary phase in a column used to isolate antibodies to SARS-CoV-2? How are the antibodies eluted off the column to isolate them for drug development?Please propose a chromatography-based strategy to purify human insulin proteins that have been expressed in bacterial cells.
- In normal-phase HPLC chromatography, the stationary phase is polar and the mobile phase is nonpolar. In the reversed phase we have just the opposite; the stationary phase is nonpolar and the mobile phase is polar. Describe molecules in which move faster or slower in each case.what concentration does surfactant polyoxyethylene sorbitan monolaurate have to make a measurement and have a range?Ј.B. is a 52-year-old man weighing 72 kg who has been treated with gentamicin for confirmed gram-negative pneumonia. Design the dose of gentamicin (given in a one-hour infusion) to achieve Cmax = 10 mg / L and Cmin = 1.6 mg / L with values for V = 18 L and t1 / 2 = 8 hours. please give me answer using pharmacokinetics!!! URGENT
- Three students did a chromatography experiment, where Rf = distance of solute / distance of solvent. What could be the possible errors why student 3 had results that are quite far from that of students A and B?Use the following information collected from a SIZE-EXCLUSION CHROMATOGRAPHY experiment to answer the questions below. MW Standards Molecular Weight (kDa) Elution Volume (mL) blue dextran 1000 85 myoglobin 17.2 200 TIM 53.3 178 hemoglobin 62.2 156 IgG 140 122 ATCase 307.9 94 Prepare a graph showing the linear relationship between log function of molecular weight against Ve/Vo. The R2value must be 0.9 or higher. What is the linear equation from the standards given? What is the R2value?Suppose a difference in changes in plasma oxytocin levels between two groups of 0.2 pg/mL would be of interest to researchers and that changes within each group have standard deviation o = 0.1 pg/mL. The power of a one-sided two-sample t-test to detect a difference in changes in oxytocin levels of 0.2 pg/mL between two groups of 5 women at the 5% level is
- monolayers! 6. If there is one monolayer coverage of organic contamination on the wafer, how much is that counted as carbon atoms/cm2?Plot a professional quality standard curve (absorbance vs. protein amount in µg) with an appropriate title and caption underneath the plot from the data give above. The caption should include the best-fit data.i) To estimate the accuracy and precision of the method of determination of glucose in a blood sample, blood was spiked with a 88.2 ngml1 of glucose. The amount of glucose was determined in the spiked sample giving the following results in ngml1, 83.6, 69.0, 83.4 100.0 and 76.4. a)Find the accuracy of the quality control sample. b)Find the precision of the quality control sample