Prepare a standard curve from the data below from a Bradford assay. Samples were prepared from a 0.100 mg/mL egg white lysozyme stock solution in a 100.0 mM, pH 7.0 phosphate buffer at various dilutions in a final volume of 1.0 mL. The data is shown in the table below: Dilution (%) 0.0 (blank) 2.5 → 0.0025 mg 5.0 → 0.005 mg Absorbance @ 595 nm (1 cm pathlength) 0.000 0.031 0.075 10.0 → 0.01 mg 0.134 15.0 → 0.015 0.229 20.0 → 0.02 0.280 25.0 → 0.025 0.386 37.5 → 0.0375 0.540 50.0 → 0.05 mg 0.742

Prepare a standard curve from the data below from a Bradford assay. Samples were prepared from a 0.100 mg/mL egg white lysozyme stock solution in a 100.0 mM, pH 7.0 phosphate buffer at various dilutions in a final volume of 1.0 mL. The data is shown in the table below: Dilution (%) 0.0 (blank) 2.5 → 0.0025 mg 5.0 → 0.005 mg Absorbance @ 595 nm (1 cm pathlength) 0.000 0.031 0.075 10.0 → 0.01 mg 0.134 15.0 → 0.015 0.229 20.0 → 0.02 0.280 25.0 → 0.025 0.386 37.5 → 0.0375 0.540 50.0 → 0.05 mg 0.742

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter15: Molecular Luminescence Spectrometry

Section: Chapter Questions

Problem 15.10QAP

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Question

Plot a professional quality standard curve (absorbance vs. protein amount in µg) with an appropriate title and caption underneath the plot from the data give above. The caption should include the best-fit data.

Prepare a standard curve from the data below from a Bradford assay. Samples were
prepared from a 0.100 mg/mL egg white lysozyme stock solution in a 100.0 mM, pH 7.0
phosphate buffer at various dilutions in a final volume of 1.0 mL. The data is shown in the
table below:
Dilution (%)
Absorbance @ 595 nm (1 cm pathlength)
0.0 (blank)
2.5 → 0.0025 mg
5.0 → 0.005 mg
0.000
0.031
0.075
10.0 → 0.01 mg
0.134
15.0 → 0.015
0.229
20.0 → 0.02
0.280
25.0 → 0.025
0.386
37.5 → 0.0375
0.540
50.0 → 0.05 mg
0.742

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