A purple solution is prepared by mixing 5.00 mL of the blue dye solution with 6.25 mL of red dye solution. The calibration curve for each color is provided. Measurement Result Measurement Blue dye stock solution (M) Result 0.1944 M Red dye stock solution (M) 0.2015 M Absorbance at 630 nm 0.00697 0.00193 Absorbance at 500 nm Calibration curve Calibration curve y=0.0682x y=0.0170x Part: 0/4 Part 1 of 4 Determine the theoretical molar concentration of each dye in the mixture. Round your answer to 3 significant digits. [Blue theoretical M [Red theoretical M X Part: 1/4 Part 2 of 4 Using the absorbance and calibration curve for each dye, calculate the experimental molar concentration of each dye in the mixture. Round your answer to 3 significant digits. [Blue] M E Submit Terms of Use | Privacy Center

A purple solution is prepared by mixing 5.00 mL of the blue dye solution with 6.25 mL of red dye solution. The calibration curve for each color is provided. Measurement Result Measurement Blue dye stock solution (M) Result 0.1944 M Red dye stock solution (M) 0.2015 M Absorbance at 630 nm 0.00697 0.00193 Absorbance at 500 nm Calibration curve Calibration curve y=0.0682x y=0.0170x Part: 0/4 Part 1 of 4 Determine the theoretical molar concentration of each dye in the mixture. Round your answer to 3 significant digits. [Blue theoretical M [Red theoretical M X Part: 1/4 Part 2 of 4 Using the absorbance and calibration curve for each dye, calculate the experimental molar concentration of each dye in the mixture. Round your answer to 3 significant digits. [Blue] M E Submit Terms of Use | Privacy Center

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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A solution of potassium permanganate of unknown concentration was analyzed against a standard of various concentrations. What is the concentration of KMNO4 if its absorbance is 0.678? Table 1. Data for the Standard Calibration Curve KMNO4 Standard Solution (mol/L) Absorbance at 525 nm 0.000400 0.112 0.000800 0.295 0.000120 l0.443 0.000160 0.701 0.00200 0.932 O 0.00173 M 0.00194 M O 0,00189 O 0.00155 M
After determining the absorbance of several standards of known concentration, the trendline for a calibration curve (AKA a standard curve) plotting absorbance (y-axis) against concentration (M, x-axis) is determined to be y = 4.586x + -0.0010 %3D The absorbance of a solution of unknown concentration is determined to be 0.68. Calculate the concentration of the unknown solution in M. Give your answer to thrėe decimal places.
A standard curve of absorbance vs. concentration has a linear trendline of y = 0.56x + 0.987. Use this trendline to calculate the concentration of an unknown sample that has an absorbance of 1.245.
The intensity of red light (680 nm) absorbed by a solution of green dye was found to be 1.59 V and the intensity of light when no dye was present (the blank) was found to be 2.75 V. What is the absorbance of the solution of green dye? Type only the value in the answer field. Report answer to three significant figures.
Blue Blue dye stock solution 0.293 M Absorbance at 630 nm 0.00265 Calibration curve y = 0.0833x A solution is prepared by diluting 2.81 mL of the blue dye stock solution to 25.00 mL. The measure absorbance for the prepared solution is listed in the data table. (a) What is the theoretical molar concentration? [Blue]theoretical | × 10 |M (b) What is the experimental molar concentration? [Bluelexperimental x 10 M (c) What is the percent error? Percent error (blue) =
A student weighed out 0.150 g of protein powder and dissolved it in 100 mL of water (Solution 1). The student then diluted this solution by transferring 1 mL into a 25 mL flask and diluting with water (Solution 2). Finally, 1 mL of that solution was transferred to a test tube and combined with 4 mL Bradford reagent. The absorbance of the solution in the test tube was 0.11. Assuming that the best fit linear line of the standard curve was y = 0.04144 x + 0.01521 (μ g mL), calculate the percent protein by mass in the original protein powder.
Application Questions: Answer the following questions. Show all work for calculations and include units and the correct number of significant figures. 1. A solution of cobalt (III) chloride was analyzed using a UV/Vis spectrometer and produced the spectra shown in Fig. 4. What is the best wavelength to use for analyzing the samples? Best wavelength to analyze sample: Absorbance 0.600 0.500 0.400 0.300 0.200 0.100 0.000 375 400 450 425 475 500 525 550 Wavelength (nm) Molarity of FD&C #2 0.000 0.115 0.225 0.350 575 Figure 4. UV/Vis spectra of a cobalt (III) chloride solution for determination of the best wavelength for analysis. 8 600 625 2. Create a graph in Excel and add a linear trendline using the data given below. Calculate the molarity of a solution (to 3 significant digits) with an absorbance of 0.576 using the trendline equation from this graph. Show your work in the box below. DO NOT ATTACH THE FIGURE. Abs 0.002 0.187 0.442 0.699 650 675
The best fit curve line to an absorbance vs. concentration plot for standard solutions of a dye has a slope of 0.1815 mL/mg and intercept of 0.0477. A sample solution prepared of the same dye has an absorbance of 0.464 at the wavelength used to establish the Beer's law plot. What is the concentration of dye in the prepared solution? Select one: O a. 0.132 mg/mL b. 2.29 mg/mL O c. 0.464 mg/mL O d. 2.82 mg/mL e. 0.0365 mg/mL Next page Law Tutorial Part 2 Jump to... Exp #9 - Laboratory Practical► Worksheet étv A
A solution of a dye was analyzed by spectrophotometry, and the following calibration data were collected using a 1 cm cuvette. *graph in attachemnt* Using the calibration data above for the dye solution, what is the dye concentration in a solution with an absorbance (A) = 0.52 if it was measured in a 1 cm cuvette? Group of answer choices 3.0 x 10-6 M 6.0 x 104 M 2.0 x 10-6 M Not enough information is provided.
4 of 15 Abs 2.0 1.5 1.0 0.5 0.25 0. 0.2 0.4 0.6 Concentration (M) With reference to the UV-Vis calibration curve reported above, calculate the concentration (Molar) of a sample whose Absorbance is 1.0. Report your answer with two significant figures.
After determining the absorbance of several standards of known concentration, the trendline for a calibration curve (AKA a standard curve) plotting absorbance (y-axis) against concentration (M, x-axis) is determined to be y = 4.943x + -0.0001 The absorbance of a solution of unknown concentration is determined to be 0.48. Calculate the concentration of the unknown solution in M. Give your answer to three decimal places.
Using the concentrations found in the experiment for the iron cation and thiocyanate anion determine the Kc for iron (III) thiocyanate using 1.71mL of iron cation solution and 4.00 mL of thiocyanate anion solution. The absorbance for this mixture at 460 nm is 0.339. Please answer type in word don't image upload thank you.