C. Spectrophotometry 1. Measure the absorbance of the standard solutions and construct a calibration curve. 2. Measure the absorbance of the unknown solutions and determine the concentration of iron (II) in the sample. Below are the measured absorbance of the standard concentrations and unknown samples in triplicates. Sample Absorbance 0.87 0.70 0.54- Standard 1 Standard 2 Standard 3 Standard 4 Standard 5 Standard 6 0.41 0.23 0.03 Average Concentration (pg/mL) Absorbance Unknown 1 Unknown 2 Unknown 3 T1 0.25 0.19 T2 0.24 T3 0.24 0.16 0.35 0.18 0.33 0.32
C. Spectrophotometry 1. Measure the absorbance of the standard solutions and construct a calibration curve. 2. Measure the absorbance of the unknown solutions and determine the concentration of iron (II) in the sample. Below are the measured absorbance of the standard concentrations and unknown samples in triplicates. Sample Absorbance 0.87 0.70 0.54- Standard 1 Standard 2 Standard 3 Standard 4 Standard 5 Standard 6 0.41 0.23 0.03 Average Concentration (pg/mL) Absorbance Unknown 1 Unknown 2 Unknown 3 T1 0.25 0.19 T2 0.24 T3 0.24 0.16 0.35 0.18 0.33 0.32
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Please help me with letter C table
Transcribed Image Text:deionized water.
2. Serial dilution is performed to create the standard solutions. Pipette the
appropriate volume to 50mL volumetric flask. Add distilled deionized water up
until the mark, stopper and mix the solution.
(Determine the appropriate volume by filling in the table below.)
Standard Iron(II) Solutions (ppm)
1. 250
2. 200
3. 150
4. 100
5. 50
6. 0
Volume to be pippeted (mL)
Volume from stock:
Volume of 1: 40.00
Volume of 2: 37.50
Volume of 3: 33.33
Volume of 4: 25.00
Below is the obtained spectrum.
0.9-
0.8-
| 510nm
0.7
0.6-
0.5-
0.4
0.3
02
400
500
600.
Wavelength (nm)
C. Spectrophotometry
1. Measure the absorbance of the standard solutions and construct a
Absorbance
Transcribed Image Text:Paragraph
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6
7.18 I9I
10.1
11
12
1.
13.1
14
15
16 1
18
C. Spectrophotometry
1. Measure the absorbance of the standard solutions and construct a
calibration curve.
2. Measure the absorbance of the unknown solutions and determine the
concentration of iron (II) in the sample.
Below are the measured absorbance of the standard concentrations and unknown
samples in triplicates.
Sample
Absorbance
Standard 1
Standard 2
Standard 3
Standard 4
Standard 5
Standard 6
0.87
0.70
0.54-
0.41
0.23
0.03
Average Concentration
(pg/mL)
Absorbance
T2
0.24
0.18
0.32
T1
0.25
T3
0.24
Unknown 1
Unknown 2
Unknown 3
0.16
0.35
0.19
0.33
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