Two groups in a BIO 120 lab extracted a blue-violet pigment from mayana plant which was to be used as a natural dye for Philippine textiles. To determine the concentration of the pigment isolated, each group made a standard curve using commercially available indigo dye in dimethylsulfoxide (DMSO). The following data were obtained by the two groups: Table 4. Absorbance readings of standard solutions of varying concentrations of indigo dye in DMSO. Pure DMSO 0.115 Standard solutions (ppm) Group 1 Data Group 2 Data 0.2 0.398 0.224 0.4 0.563 0.437 0.6 0.732 0.603 0.8 0.833 0.811 1.0 1.008 0.966 1. Show the constructed standard curves of the two groups with proper figure titles. 2. Which of the two groups generated a better standard curve? Justify your answer by providing the R value for each data set. 3. The extracted pigment has an absorbance of 0.983. When a 0.2 mL-aliquot of the extract was diluted to 1.0 mL, the absorbance of the diluted solution was found to be 0.311. Using data from the group that you chose in #1, compute for the/determine the following: a. absorptivity coefficient, a, from the slope of the standard curve (3 decimal places) b. actual concentration of the sample (3 decimal places) using the Lambert-Beer Law, assuming a sample path length of 1 cm.
Two groups in a BIO 120 lab extracted a blue-violet pigment from mayana plant which was to be used as a natural dye for Philippine textiles. To determine the concentration of the pigment isolated, each group made a standard curve using commercially available indigo dye in dimethylsulfoxide (DMSO). The following data were obtained by the two groups: Table 4. Absorbance readings of standard solutions of varying concentrations of indigo dye in DMSO. Pure DMSO 0.115 Standard solutions (ppm) Group 1 Data Group 2 Data 0.2 0.398 0.224 0.4 0.563 0.437 0.6 0.732 0.603 0.8 0.833 0.811 1.0 1.008 0.966 1. Show the constructed standard curves of the two groups with proper figure titles. 2. Which of the two groups generated a better standard curve? Justify your answer by providing the R value for each data set. 3. The extracted pigment has an absorbance of 0.983. When a 0.2 mL-aliquot of the extract was diluted to 1.0 mL, the absorbance of the diluted solution was found to be 0.311. Using data from the group that you chose in #1, compute for the/determine the following: a. absorptivity coefficient, a, from the slope of the standard curve (3 decimal places) b. actual concentration of the sample (3 decimal places) using the Lambert-Beer Law, assuming a sample path length of 1 cm.
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Two groups in a BIO 120 lab extracted a blue-violet pigment from mayana plant which was to be used as a
natural dye for Philippine textiles. To determine the concentration of the pigment isolated, each group
made a standard curve using commercially available indigo dye in dimethylsulfoxide (DMSO). The following
data were obtained by the two groups:
Table 4. Absorbance readings of standard solutions of varying concentrations of indigo dye in DMSO.
Pure DMSO
0.115
Standard solutions (ppm)
Group 1 Data
Group 2 Data
0.2
0.398
0.224
0.4
0.563
0.437
0.6
0.732
0.603
0.8
0.833
0.811
1.0
1.008
0.966
1. Show the constructed standard curves of the two groups with proper figure titles.
2. Which of the two groups generated a better standard curve? Justify your answer by providing the R?
value for each data set.
3. The extracted pigment has an absorbance of 0.983. When a 0.2 mL-aliquot of the extract was diluted to
1.0 mL, the absorbance of the diluted solution was found to be 0.311. Using data from the group that
you chose in #1, compute for the/determine the following:
a. absorptivity coefficient, a, from the slope of the standard curve (3 decimal places)
b. actual concentration of the sample (3 decimal places) using the Lambert-Beer Law, assuming a
sample path length of 1 cm.
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