The Keq for imidazole binding to hemoglobin can be determined from the following linear equation, derived from measured lab data. It is used mainly because we will never be directly measuring A100, as it is a theoretical limit that cannot be reached. 1 A4,00 K eq X + [Im] Δ4100 ΔΑ a. If the reciprocal of the change in absorbance (1/AA) is plotted versus the reciprocal of the imidazole concentration (1/[Im]), the slope and the y-intercept are important in interpreting
Catalysis and Enzymatic Reactions
Catalysis is the kind of chemical reaction in which the rate (speed) of a reaction is enhanced by the catalyst which is not consumed during the process of reaction and afterward it is removed when the catalyst is not used to make up the impurity in the product. The enzymatic reaction is the reaction that is catalyzed via enzymes.
Lock And Key Model
The lock-and-key model is used to describe the catalytic enzyme activity, based on the interaction between enzyme and substrate. This model considers the lock as an enzyme and the key as a substrate to explain this model. The concept of how a unique distinct key only can have the access to open a particular lock resembles how the specific substrate can only fit into the particular active site of the enzyme. This is significant in understanding the intermolecular interaction between proteins and plays a vital role in drug interaction.
please help with this textbook activity.
I need parts A,B,C please
![The Keq for imidazole binding to hemoglobin can be determined from the following linear equation,
derived from measured lab data. It is used mainly because we will never be directly measuring A100,
as it is a theoretical limit that cannot be reached.
1
1
X
+
K [Im] Δ4100 ΔΑ
100 eq
=
a.
If the reciprocal of the change in absorbance (1/AA) is plotted versus the reciprocal of the
imidazole concentration (1/[Im]), the slope and the y-intercept are important in interpreting
the result. Clearly mark a square around the term equivalent to slope and a triangle around
the y-intercept.
b. If the best-fit line for your data gave a slope of 0.208 M and an intercept of 21.3, determine
values of AA100 and Keq?
Answer in this box:
AA100=
Keq =
c. Using the calculated Keq in part b of this question and an [Im] = 0.114 M, what ratio of bound
to unbound ferrihemoglobin would you predict?
Answer in this box:](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F7a6c3af6-91f1-478b-99db-a0b0fd9518f2%2F2119ec72-e213-4650-b9ca-f56e71faf136%2Fgr4z1w_processed.jpeg&w=3840&q=75)
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