The absorption coefficient of bovine immunoglobin (molecular mass= 150,000 Daltons) at 280nm is 210,000 M^-1 cm^ -1. What is the absorbance of a 0.1 mg ml^-1 solution across a 1 cm path? What percentage of the incident light is transmitted by this solution?

The absorption coefficient of bovine immunoglobin (molecular mass= 150,000 Daltons) at 280nm is 210,000 M^-1 cm^ -1. What is the absorbance of a 0.1 mg ml^-1 solution across a 1 cm path? What percentage of the incident light is transmitted by this solution?

Principles of Modern Chemistry

8th Edition

ISBN:9781305079113

Author:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler

Publisher:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler

Chapter20: Molecular Spectroscopy And Photochemistry

Section: Chapter Questions

Problem 6P

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Spectroanalytical Methods

Spectroanalytical methods measure the wavelength and intensity of radiations that are either emitted or absorbed by a molecular or atomic species. These methods are useful for evaluating the electronic or atomic arrangement of the molecules of a given analyte.

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