13. 0 SOOO The following curve was made by absorption spectrophotometry (2= 500 nm) of standard protein solutions using the Lowry method you will perform in lab Exercise #4. If an unknown protein solution was analyzed using the same system and an absorbance value of 0.125 was measured, what was the protein concentration of the unknown? 1 0 B. Absorbance at 500 nm 0 C. 5 D. 0.8 0.6 0.4 0.2 A. 107 μg/mL 83.7 μg/mL 71.4 μg/mL 60.2 μg/mL y = 1.492E-03x + 3.513E-02 0 100 200 300 400 500 600 Protein concentration, ug/mL
13. 0 SOOO The following curve was made by absorption spectrophotometry (2= 500 nm) of standard protein solutions using the Lowry method you will perform in lab Exercise #4. If an unknown protein solution was analyzed using the same system and an absorbance value of 0.125 was measured, what was the protein concentration of the unknown? 1 0 B. Absorbance at 500 nm 0 C. 5 D. 0.8 0.6 0.4 0.2 A. 107 μg/mL 83.7 μg/mL 71.4 μg/mL 60.2 μg/mL y = 1.492E-03x + 3.513E-02 0 100 200 300 400 500 600 Protein concentration, ug/mL
Macroscale and Microscale Organic Experiments
7th Edition
ISBN:9781305577190
Author:Kenneth L. Williamson, Katherine M. Masters
Publisher:Kenneth L. Williamson, Katherine M. Masters
Chapter14: Ultraviolet Spectroscopy, Refractive Indices, And Qualitative Instrumental Organic Analysis
Section: Chapter Questions
Problem 4Q
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![13.
0
0
0
5
The following curve was made by absorption spectrophotometry (2 = 500 nm) of standard protein
solutions using the Lowry method you will perform in lab Exercise #4. If an unknown protein solution
was analyzed using the same system and an absorbance value of 0.125 was measured, what was the
protein concentration of the unknown?
1
Absorbance at 500 nm
0.8
0.6
0.4
0.2
y = 1.492E-03x + 3.513E-02
0 100
A. 107 μg/mL
B. 83.7 μg/mL
C. 71.4 μg/mL
D. 60.2 µg/mL
200 300 400 500 600
Protein concentration, ug/mL](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F735acb15-8c4c-426e-996a-2af1a2845819%2F14b9d9cf-1380-45f0-a078-3bb302d649b3%2Fa1dimjr_processed.png&w=3840&q=75)
Transcribed Image Text:13.
0
0
0
5
The following curve was made by absorption spectrophotometry (2 = 500 nm) of standard protein
solutions using the Lowry method you will perform in lab Exercise #4. If an unknown protein solution
was analyzed using the same system and an absorbance value of 0.125 was measured, what was the
protein concentration of the unknown?
1
Absorbance at 500 nm
0.8
0.6
0.4
0.2
y = 1.492E-03x + 3.513E-02
0 100
A. 107 μg/mL
B. 83.7 μg/mL
C. 71.4 μg/mL
D. 60.2 µg/mL
200 300 400 500 600
Protein concentration, ug/mL
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