a. The graph shows that the basic form has a maximum absorption at Amax, while the acidic has its minimum absorption at Amax. Explain why the basic form and acidic form have opposite absorptions at this specific wavelength. b. What is the importance of determining the Amax?

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2. For the first part of the lab, the following graph was produced.
0.6
Absorbance Curves for BCG
basic forn
bacidic form
0.5
0.4
0.3
0.2-
0.1
0.0
350
4505
500
550
600
650
700
400
wavelength (nm)
max: Wavelength of maximum absorbance for base form
a. The graph shows that the basic form has a maximum absorption at Amax, while the acidic has
its minimum absorption at Amax. Explain why the basic form and acidic form have opposite
absorptions at this specific wavelength.
b. What is the importance of determining the Amax?
Mixed (absorbance)
Transcribed Image Text:2. For the first part of the lab, the following graph was produced. 0.6 Absorbance Curves for BCG basic forn bacidic form 0.5 0.4 0.3 0.2- 0.1 0.0 350 4505 500 550 600 650 700 400 wavelength (nm) max: Wavelength of maximum absorbance for base form a. The graph shows that the basic form has a maximum absorption at Amax, while the acidic has its minimum absorption at Amax. Explain why the basic form and acidic form have opposite absorptions at this specific wavelength. b. What is the importance of determining the Amax? Mixed (absorbance)
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