explain what the results of this experiment are and the purpose Table 2 Absorbance vs CoCl2 concentration Test Tube Number Cobalt Chloride Concentration (mol/mL) Absorbance at 510 nm 1 0.000 0.000 2 0.009 0.024 3 0.018 0.055 4 0.027 0.085 5 6 0.036 0.045 0.112 0.138 7 (Unknown) 0.088

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explain what the results of this experiment are and the purpose

Table 2   Absorbance vs CoCl2 concentration

 

Test Tube Number

Cobalt Chloride Concentration    (mol/mL)

Absorbance at

510 nm

 

 

 

1

0.000

0.000

2

0.009

0.024

3

0.018

0.055

4

0.027

0.085

5

6

0.036

0.045

0.112

0.138

 

7

 

(Unknown)

 

0.088

 

Add 5.0 mL of the unknown cobalt chloride to test tube 7.
2.
3. Cover test tubes 2-5 with parafilm and mix using a vortex mixer.
Set the wavelength on the spectrophotometer to the absorption peak found in procedure 1.
4.
Using a clean plastic transfer pipette for each of the solutions, add 3.0 mL of solution from
5.
each test tube into separate cuvettes.
6. Blank spectrophotometer using cuvette 1 by pressing "0/ABS."
7.
Put the remaining cuvettes in the spectrophotometer and record the absorbances of each
cuvette in the table below. There is no need to blank the spectrophotometer before each
cuvette since the wavelength is not being changed.
8. Compare the absorbance to the standard cobalt chloride curve and read the concentration
directly from the graph as shown in the introduction.
9. Pour the cobalt chloride solutions into the labeled waste bottle.
Cobalt Chloride
Test Tube Number
Absorbance at 510 nm
Concentration
(mol/mL)
1
0.000
0024
0.009
0.024
0.018
0.055
0.027
#
ตาร
0.015
0.112.
0.036
0.045
0-138
M (Unknown)
COTE
2
3
4
5
6
7
460
Transcribed Image Text:Add 5.0 mL of the unknown cobalt chloride to test tube 7. 2. 3. Cover test tubes 2-5 with parafilm and mix using a vortex mixer. Set the wavelength on the spectrophotometer to the absorption peak found in procedure 1. 4. Using a clean plastic transfer pipette for each of the solutions, add 3.0 mL of solution from 5. each test tube into separate cuvettes. 6. Blank spectrophotometer using cuvette 1 by pressing "0/ABS." 7. Put the remaining cuvettes in the spectrophotometer and record the absorbances of each cuvette in the table below. There is no need to blank the spectrophotometer before each cuvette since the wavelength is not being changed. 8. Compare the absorbance to the standard cobalt chloride curve and read the concentration directly from the graph as shown in the introduction. 9. Pour the cobalt chloride solutions into the labeled waste bottle. Cobalt Chloride Test Tube Number Absorbance at 510 nm Concentration (mol/mL) 1 0.000 0024 0.009 0.024 0.018 0.055 0.027 # ตาร 0.015 0.112. 0.036 0.045 0-138 M (Unknown) COTE 2 3 4 5 6 7 460
Wavelength (nm)
400
410
420
430
440
450
460
470
480
490
500
510
520
530
540
550
560
570
580
590
600
610
620
630
640
650
660
670
680
690
700
7
7
Absorbance
0.035
0.043
олоо
0.091
0.143
0. 269
0.321
0.304
0.398
0 445
toe'o
ольо
0.452
0.395
0.297
0.005
0.137
0500
о.оча
seo'o
геото
②
0.018
о.оде
0.62
ов
0.013
чтото
0.064
DOIL
2.8
602
0.003
о. ос
06|
6
PgUp
Transcribed Image Text:Wavelength (nm) 400 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 7 7 Absorbance 0.035 0.043 олоо 0.091 0.143 0. 269 0.321 0.304 0.398 0 445 toe'o ольо 0.452 0.395 0.297 0.005 0.137 0500 о.оча seo'o геото ② 0.018 о.оде 0.62 ов 0.013 чтото 0.064 DOIL 2.8 602 0.003 о. ос 06| 6 PgUp
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