The protein content of the unknown samples were determined using BSA as standard. The absorbance values of the standard solutions and samples are as follows. BSA concentration (mg/mL) Absorbance 0.000 20 0.110 40 0.192 60 0.248 80 0.325 100 0.400 120 0.460 What is the equation of the line? b. Determine the protein concentration of samples in µg/mL. Sample dilutions A540 1 in 14 1 in 5 1 in 8 0.150 0.380 0.235

The protein content of the unknown samples were determined using BSA as standard. The absorbance values of the standard solutions and samples are as follows. BSA concentration (mg/mL) Absorbance 0.000 20 0.110 40 0.192 60 0.248 80 0.325 100 0.400 120 0.460 What is the equation of the line? b. Determine the protein concentration of samples in µg/mL. Sample dilutions A540 1 in 14 1 in 5 1 in 8 0.150 0.380 0.235

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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The protein content of the unknown samples were determined using BSA as standard. The
absorbance values of the standard solutions and samples are as follows.
BSA concentration (mg/mL)
Absorbance
0.000
20
0.110
40
0.192
60
0.248
80
0.325
100
0.400
120
0.460
a. What is the equation of the line?
b. Determine the protein concentration of samples in ug/mL.
Sample dilutions
1 in 14
A540
0.150
1 in 5
1 in 8
0.380
0.235

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