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Table 2   Absorbance vs CoCl2 concentration   Test Tube Number Cobalt Chloride Concentration    (mol/mL) Absorbance at 510 nm       1 0.000 0.000 2 0.009 0.024 3 0.018 0.055 4 0.027 0.085 5 6 0.036 0.045 0.112 0.138   7   (Unknown)   0.088   explain the results of this experiment and find the unknown concentration

Table 2   Absorbance vs CoCl2 concentration   Test Tube Number Cobalt Chloride Concentration    (mol/mL) Absorbance at 510 nm       1 0.000 0.000 2 0.009 0.024 3 0.018 0.055 4 0.027 0.085 5 6 0.036 0.045 0.112 0.138   7   (Unknown)   0.088   explain the results of this experiment and find the unknown concentration

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Table 2   Absorbance vs CoCl2 concentration

 

Test Tube Number

Cobalt Chloride Concentration    (mol/mL)

Absorbance at

510 nm

 

 

 

1

0.000

0.000

2

0.009

0.024

3

0.018

0.055

4

0.027

0.085

5

6

0.036

0.045

0.112

0.138

 

7

 

(Unknown)

 

0.088

 

explain the results of this experiment and find the unknown concentration

 

Wavelength (nm) 400 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 7 7 Absorbance 0.035 0.043 олоо 0.091 0.143 0. 269 0.321 0.304 0.398 0 445 toe'o ольо 0.452 0.395 0.297 0.005 0.137 0500 о.оча seo'o геото ② 0.018 о.оде 0.62 ов 0.013 чтото 0.064 DOIL 2.8 602 0.003 о. ос 06| 6 PgUp
Transcribed Image Text:Wavelength (nm) 400 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 7 7 Absorbance 0.035 0.043 олоо 0.091 0.143 0. 269 0.321 0.304 0.398 0 445 toe'o ольо 0.452 0.395 0.297 0.005 0.137 0500 о.оча seo'o геото ② 0.018 о.оде 0.62 ов 0.013 чтото 0.064 DOIL 2.8 602 0.003 о. ос 06| 6 PgUp
Add 5.0 mL of the unknown cobalt chloride to test tube 7. 2. 3. Cover test tubes 2-5 with parafilm and mix using a vortex mixer. Set the wavelength on the spectrophotometer to the absorption peak found in procedure 1. 4. Using a clean plastic transfer pipette for each of the solutions, add 3.0 mL of solution from 5. each test tube into separate cuvettes. 6. Blank spectrophotometer using cuvette 1 by pressing "0/ABS." 7. Put the remaining cuvettes in the spectrophotometer and record the absorbances of each cuvette in the table below. There is no need to blank the spectrophotometer before each cuvette since the wavelength is not being changed. 8. Compare the absorbance to the standard cobalt chloride curve and read the concentration directly from the graph as shown in the introduction. 9. Pour the cobalt chloride solutions into the labeled waste bottle. Cobalt Chloride Test Tube Number Absorbance at 510 nm Concentration (mol/mL) 1 0.000 0024 0.009 0.024 0.018 0.055 0.027 # ตาร 0.015 0.112. 0.036 0.045 0-138 M (Unknown) COTE 2 3 4 5 6 7 460
Transcribed Image Text:Add 5.0 mL of the unknown cobalt chloride to test tube 7. 2. 3. Cover test tubes 2-5 with parafilm and mix using a vortex mixer. Set the wavelength on the spectrophotometer to the absorption peak found in procedure 1. 4. Using a clean plastic transfer pipette for each of the solutions, add 3.0 mL of solution from 5. each test tube into separate cuvettes. 6. Blank spectrophotometer using cuvette 1 by pressing "0/ABS." 7. Put the remaining cuvettes in the spectrophotometer and record the absorbances of each cuvette in the table below. There is no need to blank the spectrophotometer before each cuvette since the wavelength is not being changed. 8. Compare the absorbance to the standard cobalt chloride curve and read the concentration directly from the graph as shown in the introduction. 9. Pour the cobalt chloride solutions into the labeled waste bottle. Cobalt Chloride Test Tube Number Absorbance at 510 nm Concentration (mol/mL) 1 0.000 0024 0.009 0.024 0.018 0.055 0.027 # ตาร 0.015 0.112. 0.036 0.045 0-138 M (Unknown) COTE 2 3 4 5 6 7 460
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