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Preparing and Diluting Solutions Data Table Test Tube 1* 2 4 10ML/3.8mL 2.4mL SmL 1.53ML Concentration of Stock Solution (M,) 150 MISOM ISOM.ISOM.ISOM 10mL 10mL 10mL0 mL Concentration of Diluted Solution (M,)10 M :057M.036 MI.o3 M.02295M Volume of Stock Solution (V,) Final Volume of Diluted Solution (V,) ||0 m L Color Comparison (Rank solutions from lightest blue = 1, deepest blue = 5) 5324 1.889 .940,326 Absorbance at 635 nm .796 ,470 *Test tube #1 contains the initial stock solution. Post-Lab Questions (Use a separate sheet of paper to answer the following questions.) 1. Calculate the concentrations of the diluted solutions in test tubes #2 and 3 using Equation 2 from the Background section. 2. Complete the data table for test tubes #1, 4, and 5. Note: See the Pre-Lab Questions for the calculated values for solutions #4 and 5. 3. Compare the concentration of each solution to the color ranking. What is the relationship between the concentration of a solution and its color intensity? 4. Compare the concentration of each solution with its absorbance. What is the relationship between concentration and absorbance? 5. Obtain or prepare a graph of absorbance on the y-axis versus the concentration of each solution on the x-axis. 6. Does it make sense that the relationship between concentration and absorbance should include the origin (0,0) as a point? Explain your reasoning. 7. Based on your answer to Question #6, draw the "best-fit" straight line through the data points. How well does this straight line fit the data? Describe the accuracy of the relation- ship between concentration and absorbance. 8. The absorbance of a copper(II) sulfate solution of unknown concentration was measured by colorimetry and found to be 0,250. Use your graph of absorbance versus concentration to estimate the concentration of this unknown solution.

Preparing and Diluting Solutions Data Table Test Tube 1* 2 4 10ML/3.8mL 2.4mL SmL 1.53ML Concentration of Stock Solution (M,) 150 MISOM ISOM.ISOM.ISOM 10mL 10mL 10mL0 mL Concentration of Diluted Solution (M,)10 M :057M.036 MI.o3 M.02295M Volume of Stock Solution (V,) Final Volume of Diluted Solution (V,) ||0 m L Color Comparison (Rank solutions from lightest blue = 1, deepest blue = 5) 5324 1.889 .940,326 Absorbance at 635 nm .796 ,470 *Test tube #1 contains the initial stock solution. Post-Lab Questions (Use a separate sheet of paper to answer the following questions.) 1. Calculate the concentrations of the diluted solutions in test tubes #2 and 3 using Equation 2 from the Background section. 2. Complete the data table for test tubes #1, 4, and 5. Note: See the Pre-Lab Questions for the calculated values for solutions #4 and 5. 3. Compare the concentration of each solution to the color ranking. What is the relationship between the concentration of a solution and its color intensity? 4. Compare the concentration of each solution with its absorbance. What is the relationship between concentration and absorbance? 5. Obtain or prepare a graph of absorbance on the y-axis versus the concentration of each solution on the x-axis. 6. Does it make sense that the relationship between concentration and absorbance should include the origin (0,0) as a point? Explain your reasoning. 7. Based on your answer to Question #6, draw the "best-fit" straight line through the data points. How well does this straight line fit the data? Describe the accuracy of the relation- ship between concentration and absorbance. 8. The absorbance of a copper(II) sulfate solution of unknown concentration was measured by colorimetry and found to be 0,250. Use your graph of absorbance versus concentration to estimate the concentration of this unknown solution.

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
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Looking at the graph and the data provided, answer the questions 6-8.
# Preparing and Diluting Solutions ## Data Table | Test Tube | 1* | 2 | 3 | 4 | 5 | |-----------------|-----|------|------|-----|------| | Volume of Stock Solution (V₁) | 10 mL | 3.8 mL | 2.4 mL | 5 mL | 1.53 mL | | Concentration of Stock Solution (M₁) | 1.150 M | 1.150 M | 1.150 M | 1.150 M | 1.150 M | | Final Volume of Diluted Solution (V₂) | 10 mL | 10 mL | 10 mL | 10 mL | 10 mL | | Concentration of Diluted Solution (M₂) | 0.150 M | 0.057 M | 0.036 M | 0.075 M | 0.02295 M | | Color Comparison (Rank solutions from lightest blue = 1, deepest blue = 5) | 5 | 3 | 2 | 4 | 1 | | Absorbance at 635 nm | 1.889 | 0.796 | 0.470 | 0.940 | 0.326 | *Test tube #1 contains the initial stock solution. --- ## Post-Lab Questions (Use a separate sheet of paper to answer the following questions.) 1. **Calculate the concentrations of the diluted solutions in test tubes #2 and 3 using Equation 2 from the Background section.** 2. **Complete the data table for test tubes #1, 4, and 5.** *Note: See the Pre-Lab Questions for the calculated values for solutions #4 and 5.* 3. **Compare the concentration of each solution to the color ranking.** What is the relationship between the concentration of a solution and its color intensity? 4. **Compare the concentration of each solution with its absorbance.** What is the relationship between concentration and absorbance? 5. **Obtain or prepare a graph of absorbance on the y-axis versus the concentration of each solution on the x-axis.** 6. **Does it make sense that the relationship between concentration and absorbance should include the origin (0
Transcribed Image Text:# Preparing and Diluting Solutions ## Data Table | Test Tube | 1* | 2 | 3 | 4 | 5 | |-----------------|-----|------|------|-----|------| | Volume of Stock Solution (V₁) | 10 mL | 3.8 mL | 2.4 mL | 5 mL | 1.53 mL | | Concentration of Stock Solution (M₁) | 1.150 M | 1.150 M | 1.150 M | 1.150 M | 1.150 M | | Final Volume of Diluted Solution (V₂) | 10 mL | 10 mL | 10 mL | 10 mL | 10 mL | | Concentration of Diluted Solution (M₂) | 0.150 M | 0.057 M | 0.036 M | 0.075 M | 0.02295 M | | Color Comparison (Rank solutions from lightest blue = 1, deepest blue = 5) | 5 | 3 | 2 | 4 | 1 | | Absorbance at 635 nm | 1.889 | 0.796 | 0.470 | 0.940 | 0.326 | *Test tube #1 contains the initial stock solution. --- ## Post-Lab Questions (Use a separate sheet of paper to answer the following questions.) 1. **Calculate the concentrations of the diluted solutions in test tubes #2 and 3 using Equation 2 from the Background section.** 2. **Complete the data table for test tubes #1, 4, and 5.** *Note: See the Pre-Lab Questions for the calculated values for solutions #4 and 5.* 3. **Compare the concentration of each solution to the color ranking.** What is the relationship between the concentration of a solution and its color intensity? 4. **Compare the concentration of each solution with its absorbance.** What is the relationship between concentration and absorbance? 5. **Obtain or prepare a graph of absorbance on the y-axis versus the concentration of each solution on the x-axis.** 6. **Does it make sense that the relationship between concentration and absorbance should include the origin (0
**Graph Analysis: Absorbance vs. Concentration** The graph presented is a depiction of absorbance plotted on the Y-axis against the concentration of solutions on the X-axis. ### Details: - **X-axis (Horizontal):** Represents the concentration of the solution in molarity (M), ranging from 0.00 to 0.16 M. - **Y-axis (Vertical):** Represents the absorbance measured in absorbance units (nm), ranging from 0.0 to 2.0. ### Graph Explanation: The line graph displays a positive correlation between the concentration of the solution and its absorbance. As the concentration increases, the absorbance also rises, suggesting a linear relationship. This type of graph is typically used in spectrophotometry to determine the concentration of an unknown solution by measuring its absorbance. **Educational Context:** This graph can be used to teach students about Beer’s Law, which states that absorbance is directly proportional to concentration in dilute solutions. This principle is fundamental in chemistry, particularly in analytical chemistry for quantifying substances in solution.
Transcribed Image Text:**Graph Analysis: Absorbance vs. Concentration** The graph presented is a depiction of absorbance plotted on the Y-axis against the concentration of solutions on the X-axis. ### Details: - **X-axis (Horizontal):** Represents the concentration of the solution in molarity (M), ranging from 0.00 to 0.16 M. - **Y-axis (Vertical):** Represents the absorbance measured in absorbance units (nm), ranging from 0.0 to 2.0. ### Graph Explanation: The line graph displays a positive correlation between the concentration of the solution and its absorbance. As the concentration increases, the absorbance also rises, suggesting a linear relationship. This type of graph is typically used in spectrophotometry to determine the concentration of an unknown solution by measuring its absorbance. **Educational Context:** This graph can be used to teach students about Beer’s Law, which states that absorbance is directly proportional to concentration in dilute solutions. This principle is fundamental in chemistry, particularly in analytical chemistry for quantifying substances in solution.
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